Recombinant
RabMAb

Anti-Proteasome Activator Subunit 4 antibody [EPR13576(B)] - N-terminal (ab181128)

Overview

  • Product name
    Anti-Proteasome Activator Subunit 4 antibody [EPR13576(B)] - N-terminal
    See all Proteasome Activator Subunit 4 primary antibodies
  • Description
    Rabbit monoclonal [EPR13576(B)] to Proteasome Activator Subunit 4 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Proteasome Activator Subunit 4 aa 1-100. The exact sequence is proprietary.
    Database link: Q14997

  • Positive control
    • HepG2, 293T, Jurkat, HeLa and K562 lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR13576(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/210.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 211 kDa).

Target

  • Function
    Activates proteasomal cleavage of peptides in an energy-independent manner. May be involved in spermatogenesis. May be involved in DNA repair.
  • Sequence similarities
    Contains 6 HEAT repeats.
  • Cellular localization
    Nucleus. Nucleus speckle. Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2).
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0077 antibody
    • PA200 antibody
    • Proteasome Activator 200 kDa antibody
    • Proteasome activator complex subunit 4 antibody
    • Proteasome activator PA200 antibody
    • PSME 4 antibody
    • psme4 antibody
    • PSME4_HUMAN antibody
    see all

Images

  • Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling Proteasome Activator Subunit 4 with ab181128 at 1/210 dilution (red) or a Rabbit monoclonal IgG (negative) (green) followed by Goat anti rabbit IgG (FITC) secondary used at 1/150 dilution.

  • All lanes : Anti-Proteasome Activator Subunit 4 antibody [EPR13576(B)] - N-terminal (ab181128) at 1/10000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : 293T lysate
    Lane 3 : Jurkat lysate
    Lane 4 : HeLa lysate
    Lane 5 : K562 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 211 kDa

References

ab181128 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181128.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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