Overview

  • Product name

    Anti-Proteasome Activator Subunit 4/PSME4 antibody
    See all Proteasome Activator Subunit 4/PSME4 primary antibodies
  • Description

    Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Immunogen

    Recombinant fragment corresponding to Human Proteasome Activator Subunit 4/PSME4 aa 105-198.
    Database link: Q14997

  • Positive control

    • IHC-P: Human heart tissue and prostate cancer tissues. WB: K562 cell lysate
  • General notes

     This product was previously labelled as Proteasome Activator Subunit 4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000.
WB Use a concentration of 4.5 µg/ml. Predicted molecular weight: 211 kDa.

Target

  • Function

    Activates proteasomal cleavage of peptides in an energy-independent manner. May be involved in spermatogenesis. May be involved in DNA repair.
  • Sequence similarities

    Contains 6 HEAT repeats.
  • Cellular localization

    Nucleus. Nucleus speckle. Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2).
  • Information by UniProt
  • Alternative names

    • KIAA0077 antibody
    • PA200 antibody
    • Proteasome Activator 200 kDa antibody
    • Proteasome activator complex subunit 4 antibody
    • Proteasome activator PA200 antibody
    • PSME 4 antibody
    • psme4 antibody
    • PSME4_HUMAN antibody
    see all

Images

  • Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab235038) at 4.5 µg/ml + K562 cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 211 kDa

  • Paraffin-embedded human prostate cancer tissue stained for Proteasome Activator Subunit 4/PSME4 using ab235038 at 1/600 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human heart tissue stained for Proteasome Activator Subunit 4/PSME4 using ab235038 at 1/600 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab235038 has not yet been referenced specifically in any publications.

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