Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13576(B)] to Proteasome Activator Subunit 4/PSME4 - N-terminal
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Product nameAnti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13576(B)] - N-terminal
See all Proteasome Activator Subunit 4/PSME4 primary antibodies
DescriptionRabbit monoclonal [EPR13576(B)] to Proteasome Activator Subunit 4/PSME4 - N-terminal
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Proteasome Activator Subunit 4/PSME4 aa 1-100. The exact sequence is proprietary.
Database link: Q14997
- HepG2, 293T, Jurkat, HeLa and K562 lysates; Jurkat cells.
This product was previously labelled as Proteasome Activator Subunit 4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab181128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 211 kDa).|
FunctionActivates proteasomal cleavage of peptides in an energy-independent manner. May be involved in spermatogenesis. May be involved in DNA repair.
Sequence similaritiesContains 6 HEAT repeats.
Cellular localizationNucleus. Nucleus speckle. Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2).
- Information by UniProt
- KIAA0077 antibody
- PA200 antibody
- Proteasome Activator 200 kDa antibody
Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling Proteasome Activator Subunit 4/PSME4 with ab181128 at 1/210 dilution (red) or a Rabbit monoclonal IgG (negative) (green) followed by Goat anti rabbit IgG (FITC) secondary used at 1/150 dilution.
All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13576(B)] - N-terminal (ab181128) at 1/10000 dilution
Lane 1 : HepG2 lysate
Lane 2 : 293T lysate
Lane 3 : Jurkat lysate
Lane 4 : HeLa lysate
Lane 5 : K562 lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 211 kDa
ab181128 has not yet been referenced specifically in any publications.