RecombinantRabMAb

Recombinant Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)
  • Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13577(B)] to Proteasome Activator Subunit 4/PSME4 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free
    See all Proteasome Activator Subunit 4/PSME4 primary antibodies

  • Description

    Rabbit monoclonal [EPR13577(B)] to Proteasome Activator Subunit 4/PSME4 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Proteasome Activator Subunit 4/PSME4 aa 1750 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14997

  • Positive control

    • IHC-P: Human liver tissue.

  • General notes

    ab238996 is the carrier-free version of ab181203 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab238996 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Proteasome Activator Subunit 4

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13577(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 200 kDa (predicted molecular weight: 211 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Proteasome Activator Subunit 4/PSME4 with unpurified ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181203).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling unpurified  Proteasome Activator Subunit 4/PSME4 with ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181203).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Proteasome Activator Subunit 4/PSME4 with purified ab181203 at 1:1000 dilution (0.59 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab181203).

  • Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)
    Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - BSA and Azide free (ab238996)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab238996? Please let us know so that we can cite the reference in this datasheet.

ab238996 has not yet been referenced specifically in any publications.

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