Recombinant Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13577(B)] to Proteasome Activator Subunit 4/PSME4 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal
See all Proteasome Activator Subunit 4/PSME4 primary antibodies -
Description
Rabbit monoclonal [EPR13577(B)] to Proteasome Activator Subunit 4/PSME4 - C-terminal -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C6, 293T, RAW264.7, HeLa and Jurkat cells; K562, HepG2 and HeLa cell lysates; IHC-P: Human liver, Human pancreas and cardiac muscle tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13577(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 200 kDa (predicted molecular weight: 211 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/50 - 1/100. |
Notes |
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WB
1/1000. Detects a band of approximately 200 kDa (predicted molecular weight: 211 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/50 - 1/100. |
Target
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Function
Activates proteasomal cleavage of peptides in an energy-independent manner. May be involved in spermatogenesis. May be involved in DNA repair. -
Sequence similarities
Contains 6 HEAT repeats. -
Cellular localization
Nucleus. Nucleus speckle. Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2). - Information by UniProt
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Database links
- Entrez Gene: 23198 Human
- Entrez Gene: 103554 Mouse
- Entrez Gene: 498433 Rat
- Omim: 607705 Human
- SwissProt: Q14997 Human
- SwissProt: Q5SSW2 Mouse
- Unigene: 413801 Human
- Unigene: 481953 Mouse
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Alternative names
- KIAA0077 antibody
- PA200 antibody
- Proteasome Activator 200 kDa antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Proteasome Activator Subunit 4/PSME4 with purified ab181203 at 1:1000 dilution (0.59 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203) at 0.6 µg/ml
Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 211 kDa
Blocking and diluting buffer: 5% NFDM/TBST -
All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203) at 1/1000 dilution (Unpurified)
Lane 1 : K562 cell line lysate
Lane 2 : HeLa cell line lysate
Lane 3 : HepG2 cell line lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 211 kDa -
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling unpurified Proteasome Activator Subunit 4/PSME4 with ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Proteasome Activator Subunit 4/PSME4 with unpurified ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab181203 has been referenced in 1 publication.
- Pecori F et al. Site-specific O-GlcNAcylation of Psme3 maintains mouse stem cell pluripotency by impairing P-body homeostasis. Cell Rep 36:109361 (2021). PubMed: 34260942