Recombinant
RabMAb

Recombinant Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)

Submit a review Submit a question

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13577(B)] to Proteasome Activator Subunit 4/PSME4 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal
    See all Proteasome Activator Subunit 4/PSME4 primary antibodies

  • Description

    Rabbit monoclonal [EPR13577(B)] to Proteasome Activator Subunit 4/PSME4 - C-terminal

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human Proteasome Activator Subunit 4/PSME4 aa 1750 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14997

  • Positive control

    • WB: C6, 293T, RAW264.7, HeLa and Jurkat cells; K562, HepG2 and HeLa cell lysates; IHC-P: Human liver, Human pancreas and cardiac muscle tissues.

  • General notes

     

     

     This product was previously labelled as Proteasome Activator Subunit 4

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13577(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 200 kDa (predicted molecular weight: 211 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/50 - 1/100.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Proteasome Activator Subunit 4/PSME4 with purified ab181203 at 1:1000 dilution (0.59 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203) at 0.6 µg/ml

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 211 kDa



    Blocking and diluting buffer: 5% NFDM/TBST
  • Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203) at 1/1000 dilution (Unpurified)

    Lane 1 : K562 cell line lysate
    Lane 2 : HeLa cell line lysate
    Lane 3 : HepG2 cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 211 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling unpurified  Proteasome Activator Subunit 4/PSME4 with ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Proteasome Activator Subunit 4/PSME4 with unpurified ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

References

Publishing research using ab181203? Please let us know so that we can cite the reference in this datasheet.

ab181203 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab181203.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com