Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13577(B)] to Proteasome Activator Subunit 4/PSME4 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal
See all Proteasome Activator Subunit 4/PSME4 primary antibodies
DescriptionRabbit monoclonal [EPR13577(B)] to Proteasome Activator Subunit 4/PSME4 - C-terminal
SpecificityThe mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
within Human Proteasome Activator Subunit 4/PSME4 aa 1750 to the C-terminus. The exact sequence is proprietary.
Database link: Q14997
- WB: C6, 293T, RAW264.7, HeLa and Jurkat cells; K562, HepG2 and HeLa cell lysates; IHC-P: Human liver, Human pancreas and cardiac muscle tissues.
This product was previously labelled as Proteasome Activator Subunit 4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab181203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 200 kDa (predicted molecular weight: 211 kDa).|
|IHC-P||1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
For unpurified use at 1/50 - 1/100.
FunctionActivates proteasomal cleavage of peptides in an energy-independent manner. May be involved in spermatogenesis. May be involved in DNA repair.
Sequence similaritiesContains 6 HEAT repeats.
Cellular localizationNucleus. Nucleus speckle. Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2).
- Information by UniProt
- KIAA0077 antibody
- PA200 antibody
- Proteasome Activator 200 kDa antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Proteasome Activator Subunit 4/PSME4 with purified ab181203 at 1:1000 dilution (0.59 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203) at 0.6 µg/ml
Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 211 kDa
Blocking and diluting buffer: 5% NFDM/TBST
All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203) at 1/1000 dilution (Unpurified)
Lane 1 : K562 cell line lysate
Lane 2 : HeLa cell line lysate
Lane 3 : HepG2 cell line lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 211 kDa
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling unpurified Proteasome Activator Subunit 4/PSME4 with ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Proteasome Activator Subunit 4/PSME4 with unpurified ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
ab181203 has not yet been referenced specifically in any publications.