RecombinantRabMAb

Recombinant Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
  • Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
  • Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
  • Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13577(B)] to Proteasome Activator Subunit 4/PSME4 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal
    See all Proteasome Activator Subunit 4/PSME4 primary antibodies

  • Description

    Rabbit monoclonal [EPR13577(B)] to Proteasome Activator Subunit 4/PSME4 - C-terminal

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human Proteasome Activator Subunit 4/PSME4 aa 1750 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14997

  • Positive control

    • WB: C6, 293T, RAW264.7, HeLa and Jurkat cells; K562, HepG2 and HeLa cell lysates; IHC-P: Human liver, Human pancreas and cardiac muscle tissues.

  • General notes

     

     

     This product was previously labelled as Proteasome Activator Subunit 4

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13577(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 200 kDa (predicted molecular weight: 211 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/50 - 1/100.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Proteasome Activator Subunit 4/PSME4 with purified ab181203 at 1:1000 dilution (0.59 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203) at 0.6 µg/ml

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 211 kDa



    Blocking and diluting buffer: 5% NFDM/TBST
  • Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203) at 1/1000 dilution (Unpurified)

    Lane 1 : K562 cell line lysate
    Lane 2 : HeLa cell line lysate
    Lane 3 : HepG2 cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 211 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling unpurified  Proteasome Activator Subunit 4/PSME4 with ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Proteasome Activator Subunit 4/PSME4 with unpurified ab181203 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)
    Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13577(B)] - C-terminal (ab181203)

References (0)

Publishing research using ab181203? Please let us know so that we can cite the reference in this datasheet.

ab181203 has not yet been referenced specifically in any publications.

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