• Product name
    Proteasome Activity Assay Kit
  • Sample type
    Cell Lysate
  • Assay type
    Enzyme activity
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    Proteasome Activity Assay Kit (ab107921) takes advantage of the chymotrypsin-like activity, utilizing an AMC-tagged peptide substrate (Proteasome Substrate (Succ-LLVY-AMC in DMSO), which releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also includes a positive control (Jurkat Cell lysate with significant proteasome activity) and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes. This permits differentiation of proteasome activity from other protease activity which may be present in samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Proteasomes are very large (20S, 26S) protein assemblies found in both the nucleus and cytoplasm of all eucaryotes (and in some procaryotes). They are responsible for the degradation and recycling of proteins which have been previously tagged with ubiquitin. Such tagged proteins are degraded into peptides approximately 7-8 amino acids long which are subsequently further degraded. The 20S assembly is the functional protease structure with chymotrypsin-like, trypsin-like and caspase-like protease activities



  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Proteasome Activity measured in Jurkat lysate

  • Proteasome Activity measured in HeLa lysate

  • Proteasome Activity measured in cell lysates showing activity (mU) per 1 mln cells.

    Samples with the concentration of 3.3e6 cells/mL were used. 50 μl of each sample was used.



This product has been referenced in:
  • Sambri I  et al. Lysosomal dysfunction disrupts presynaptic maintenance and restoration of presynaptic function prevents neurodegeneration in lysosomal storage diseases. EMBO Mol Med 9:112-132 (2017). Read more (PubMed: 27881461) »
  • Mi X  et al. Xanthohumol induces paraptosis of leukemia cells through p38 mitogen activated protein kinase signaling pathway. Oncotarget 8:31297-31304 (2017). Read more (PubMed: 28415750) »

See all 10 Publications for this product

Customer reviews and Q&As

Proteasome activity in rat muscle

Good Good 4/5 (Ease of Use)
Soleus muscle was used.10mg of muscle was homogenized in 100µl of 0.5% NP-40, and 20µl of sample was used in duplicate in the plate.

Mr. Baptiste Jude

Verified customer

Submitted Oct 07 2016

Start with 1 - 2 x 1e6 cells. Suspend the cell pellet in 500 μl (˜4 volumes) of the 0.5% NP40 solution on ice. Homogenize with a Dounce homogenizer (10 - 50 strokes) or equivalent on ice until efficient lysis is confirmed by viewing the cells under the...

Read More

Thank you for your inquiry.

We do not recommend using samples prepared with RIPA buffer because the SDS tends to interfere with the enzyme.

Addition of protease inhibitors for this assay (ab107921: Proteasome Activity Assay Kit) actually works well; since they will inhibit any proteases from degrading the proteins in the proteasome complex.

Please use a white opaque plate. The troubleshooting guide (which recommends using a black plate for fluorescence) is just a general one.

Thank you for contacting us.

I am pleased to answer your questions regarding the Proteasome Activity Assay Kit (ab107921).

1. I can confirm that it is necessary to create a new standard curve for every performed experiment. This ...

Read More

Thank you very much for your email.

As my colleague is not in the office today, I have looked into your inquiry.

I am happy to confirm that you can use any two time points as long as they are in the linear range. The difference betw...

Read More

The standard graph looks good. Based on that,you are right in saying that the samples can be diluted further maybe 1:5 this time for better time points.
From this run, assuming that the X axis is time, I think maybe 8 and 10 (units unknown) would f...

Read More

Could you please send the standard curve for the attached plot?
My colleague would be able to help with the T1 and T2 looking at the curve.

Alternatively, this is the way you could decide the time points:
It is essential to read R...

Read More

I just wanted to let you know that I am still working on your inquiry. I have contacted the originator of this product for the information you requested, and I am awaiting a reply.

I am very sorry for the delay. I will forward to you the infor...

Read More

1-10 of 23 Abreviews or Q&A


Sign up