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Rabbit polyclonal to Proteasome Inhibitor subunit 1 PI3 1
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat
Synthetic peptide, corresponding to a region within amino acids 221-271 of Human Proteasome Inhibitor subunit 1 PI3 1 (NP_848693.2).
293T, HeLa, Jurkat whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH: 7 to 8
Concentration information loading...
Immunogen affinity purified
ab140497 was affinity purified using an epitope specific to Proteasome Inhibitor subunit 1 PI3 1 immobilized on a solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 30 kDa.
Use at 2-10 µg/mg of lysate.
Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
Belongs to the proteasome inhibitor PI31 family.
Information by UniProt
Proteasome (prosome macropain) inhibitor subunit 1 antibody
Western blot - Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab140497)
All lanes : Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab140497) at 0.4 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 30 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab140497)
ab140497 at 1 µg/ml detecting Proteasome Inhibitor subunit 1 PI3 1 in 293T whole cell lysate by WB following IP.
Lane 1: ab140497 at 6µg/mg of lysate
Lane 2: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"