Product nameAnti-Proteasome Inhibitor subunit 1 PI3 1 antibody
See all Proteasome Inhibitor subunit 1 PI3 1 primary antibodies
DescriptionRabbit polyclonal to Proteasome Inhibitor subunit 1 PI3 1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat
- 293T, HeLa, Jurkat whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab140497 was affinity purified using an epitope specific to Proteasome Inhibitor subunit 1 PI3 1 immobilized on a solid support.
Our Abpromise guarantee covers the use of ab140497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 30 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPlays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
Sequence similaritiesBelongs to the proteasome inhibitor PI31 family.
- Information by UniProt
- hPI31 antibody
- PI31 antibody
- Proteasome (prosome macropain) inhibitor subunit 1 antibody
All lanes : Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab140497) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 3 minutes
ab140497 at 1 µg/ml detecting Proteasome Inhibitor subunit 1 PI3 1 in 293T whole cell lysate by WB following IP.
Lane 1: ab140497 at 6µg/mg of lysate
Lane 2: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab140497 has not yet been referenced specifically in any publications.