Product nameAnti-Proteasome Inhibitor subunit 1 PI3 1 antibody
See all Proteasome Inhibitor subunit 1 PI3 1 primary antibodies
DescriptionRabbit polyclonal to Proteasome Inhibitor subunit 1 PI3 1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 150-200 of Human Proteasome Inhibitor subunit 1 PI3 1 (NP_848693.2).
- 293T, HeLa and Jurkat whole cell lysates
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab140527 was affinity purified using an epitope specific to Proteasome Inhibitor subunit 1 PI3 1 immobilized on a solid support.
Our Abpromise guarantee covers the use of ab140527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 30 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPlays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
Sequence similaritiesBelongs to the proteasome inhibitor PI31 family.
- Information by UniProt
- hPI31 antibody
- PI31 antibody
- Proteasome (prosome macropain) inhibitor subunit 1 antibody
All lanes : Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab140527) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Predicted band size: 30 kDa
Immunoprecipitation of Proteasome Inhibitor subunit 1 PI3 1 from 293T whole cell lysate using ab140527 at 6 µg/mg lysate (1 mg for IP; 20% of IP loaded). For blotting immunoprecipitated Proteasome Inhibitor subunit 1 PI3 1, ab140527 was used at 0.4 µg/ml.
Detection: chemiluminescence with an exposure time of 10 seconds.
ab140527 has not yet been referenced specifically in any publications.