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Rabbit polyclonal to Proteasome Inhibitor subunit 1 PI3 1
Predicted to work with:
Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 150-200 of Human Proteasome Inhibitor subunit 1 PI3 1 (NP_848693.2).
293T, HeLa and Jurkat whole cell lysates
Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH: 7-8
Concentration information loading...
Immunogen affinity purified
ab140527 was affinity purified using an epitope specific to Proteasome Inhibitor subunit 1 PI3 1 immobilized on a solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
Use at 2-10 µg/mg of lysate.
Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
Belongs to the proteasome inhibitor PI31 family.
Information by UniProt
Proteasome (prosome macropain) inhibitor subunit 1 antibody
Western blot - Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab140527)
All lanes : Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab140527) at 0.1 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Predicted band size: 30 kDa
Immunoprecipitation - Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab140527)
Immunoprecipitation of Proteasome Inhibitor subunit 1 PI3 1 from 293T whole cell lysate using ab140527 at 6 µg/mg lysate (1 mg for IP; 20% of IP loaded). For blotting immunoprecipitated Proteasome Inhibitor subunit 1 PI3 1, ab140527 was used at 0.4 µg/ml.
Detection: chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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