Anti-PSMF1 antibody (ab187200)
Key features and details
- Rabbit polyclonal to PSMF1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PSMF1 antibody
See all PSMF1 primary antibodies -
Description
Rabbit polyclonal to PSMF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human PSMF1 aa 1 to the C-terminus.
Database link: Q92530 -
Positive control
- SW480, A549 and K562 cell extracts; Mouse liver and testis tissue extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 30 kDa.
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IHC-P |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 30 kDa. |
IHC-P
1/50 - 1/200. |
Target
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Function
Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28. -
Sequence similarities
Belongs to the proteasome inhibitor PI31 family. - Information by UniProt
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Database links
- Entrez Gene: 9491 Human
- Entrez Gene: 228769 Mouse
- SwissProt: Q92530 Human
- SwissProt: Q8BHL8 Mouse
- Unigene: 471917 Human
- Unigene: 146984 Mouse
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Alternative names
- hPI31 antibody
- PI31 antibody
- Proteasome (prosome macropain) inhibitor subunit 1 antibody
see all
Images
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ab18720 Immunohistochemistry of paraffin-embedded human liver injury using PSMF1 antibody at dilution of 1:100 (x40 lens).
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All lanes : Anti-PSMF1 antibody (ab187200) at 1/500 dilution
Lane 1 : SW480 cell extract
Lane 2 : A549 cell extract
Lane 3 : K562 cell extract
Lane 4 : Mouse liver tissue extract
Lane 5 : Mouse testis tissue extract
Predicted band size: 30 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab187200 has been referenced in 1 publication.
- Corridoni D et al. NOD2 and TLR2 Signal via TBK1 and PI31 to Direct Cross-Presentation and CD8 T Cell Responses. Front Immunol 10:958 (2019). PubMed: 31114588