Overview

  • Product name
    Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody
    See all Proteasome Inhibitor subunit 1 PI3 1 primary antibodies
  • Description
    Rabbit polyclonal to Proteasome Inhibitor subunit 1 PI3 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome Inhibitor subunit 1 PI3 1 aa 111-125.
    Sequence:

    YIDAEHLGDFHRTYK


    Database link: Q92530

  • Positive control
    • HUVEC cells, Lysate of HEK-293T cells overexpressing Human PSMF1

Properties

Applications

Our Abpromise guarantee covers the use of ab213563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 30 kDa.

Target

  • Function
    Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
  • Sequence similarities
    Belongs to the proteasome inhibitor PI31 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPI31 antibody
    • PI31 antibody
    • Proteasome (prosome macropain) inhibitor subunit 1 antibody
    • Proteasome inhibitor hP131 subunit antibody
    • Proteasome inhibitor PI31 subunit antibody
    • PSMB2 antibody
    • Psmf1 antibody
    • PSMF1_HUMAN antibody
    • RP23 402M7.5 antibody
    • RP4 545L17.1 antibody
    see all

Images

  • Lanes 1-2 : Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab213563) at 1/500 dilution
    Lane 3 : Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab213563) at 1/1000 dilution

    Lanes 1 & 3 : Lysate of HEK-293T cells overexpressing human PSMF1
    Lane 2 : Lysate of HEK-293T cells overexpressing human PSMF1 with PSMF1 immunizing peptide (human, 111-125)

    Secondary
    All lanes : Goat Anti-Rabbit IgG-Peroxidase

    Developed using the ECL technique.

    Predicted band size: 30 kDa

  • Immunofluorescence analysis of HUVEC cells labeling Proteasome Inhibitor subunit 1 PI3 1 with ab213563 at 1/100 dilution. Nuclear staining with Hoescht 33342 (blue)

References

ab213563 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted May 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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