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Rabbit polyclonal to Proteasome Inhibitor subunit 1 PI3 1
Synthetic peptide corresponding to Human Proteasome Inhibitor subunit 1 PI3 1 aa 111-125.
HUVEC cells, Lysate of HEK-293T cells overexpressing Human PSMF1
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.097% Sodium azide Constituent: 99% PBS
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Predicted molecular weight: 30 kDa.
Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
Belongs to the proteasome inhibitor PI31 family.
Information by UniProt
Proteasome (prosome macropain) inhibitor subunit 1 antibody
Western blot - Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab213563)
Lanes 1-2 : Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab213563) at 1/500 dilution Lane 3 : Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab213563) at 1/1000 dilution Lanes 1 & 3 : Lysate of HEK-293T cells overexpressing human PSMF1 Lane 2 : Lysate of HEK-293T cells overexpressing human PSMF1 with PSMF1 immunizing peptide (human, 111-125) Secondary All lanes : Goat Anti-Rabbit IgG-Peroxidase Developed using the ECL technique. Predicted band size: 30 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome Inhibitor subunit 1 PI3 1 antibody (ab213563)
Immunofluorescence analysis of HUVEC cells labeling Proteasome Inhibitor subunit 1 PI3 1 with ab213563 at 1/100 dilution. Nuclear staining with Hoescht 33342 (blue)
has not yet been referenced specifically in any publications.
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