Key features and details
- Rabbit polyclonal to Proteasome maturation protein
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Proteasome maturation protein antibody
See all Proteasome maturation protein primary antibodies
DescriptionRabbit polyclonal to Proteasome maturation protein
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Orangutan
Recombinant full length protein (His-T7-tag) corresponding to Human Proteasome maturation protein aa 1-141. Expressed in E.coli. N-terminal tags.
MNARGLGSELKDSIPVTELSASGPFESHDLLRKGFSCVKNELLPSHPLEL SEKNFQLNQDKMNFSTLRNIQGLFAPLKLQMEFKAVQQVQRLPFLSSSNL SLDVLRGNDETIGFEDILNDPSQSEVMGEPHLMVEYKLGLL
Database link: Q9Y244
- WB: Recombinant human Proteasome maturation protein; HeLa cell lysate. IHC-P: Human liver and kidney tissue.
This product was previously labelled as HSPC014
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 16 kDa.|
FunctionMolecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum.
Tissue specificityStrongly expressed from the basal layer to the granular layer of healthy epidermis, whereas in KLICK patients there is a gradual decrease of expression toward the granular layer.
Involvement in diseaseDefects in POMP are the cause of keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK) [MIM:601952]. KLICK is a keratinizing disorder characterized by ichthyosis, palmoplantar keratoderma with constricting bands around fingers, flexural deformities of fingers and keratotic papules in a linear distribution on the flexural side of large joints. Histological examination of the skin of affected individuals shows hypertrophy and hyperplasia of the spinous, granular and horny epidermal layer.
Sequence similaritiesBelongs to the POMP/UMP1 family.
Cellular localizationCytoplasm > cytosol. Nucleus. Microsome membrane.
- Information by UniProt
- 2510048O06Rik antibody
- C13orf12 antibody
- Chromosome 13 open reading frame 12 antibody
Anti-Proteasome maturation protein antibody (ab232900) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 16 kDa
Formalin-fixed, paraffin-embedded human liver tissue stained for proteasome maturation protein using ab232900 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human kidney tissue stained for proteasome maturation protein using ab232900 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Anti-Proteasome maturation protein antibody (ab232900) at 2 µg/ml + Recombinant human Proteasome maturation protein
Predicted band size: 16 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232900 has not yet been referenced specifically in any publications.