Recombinant Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)
![Western blot - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)](https://www.abcam.com/ps/products/170/ab170865/Images/ab170865-170746-anti-hspc014-antibody-epr10177-western-blot.jpg "Western blot - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10177] to Proteasome maturation protein
- Suitable for: WB, IHC-P, IP, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Proteasome maturation protein antibody [EPR10177]
See all Proteasome maturation protein primary antibodies -
Description
Rabbit monoclonal [EPR10177] to Proteasome maturation protein -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, Flow Cytmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Proteasome maturation protein (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y244 -
Positive control
- HeLa, SW480, HL60 and Jurkat cell lysates; Human skin tissue; Permeabilized Jurkat cells.
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General notes
This product was previously labelled as HSPC014
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10177 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab170865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 16 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum. -
Tissue specificity
Strongly expressed from the basal layer to the granular layer of healthy epidermis, whereas in KLICK patients there is a gradual decrease of expression toward the granular layer. -
Involvement in disease
Defects in POMP are the cause of keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK) [MIM:601952]. KLICK is a keratinizing disorder characterized by ichthyosis, palmoplantar keratoderma with constricting bands around fingers, flexural deformities of fingers and keratotic papules in a linear distribution on the flexural side of large joints. Histological examination of the skin of affected individuals shows hypertrophy and hyperplasia of the spinous, granular and horny epidermal layer. -
Sequence similarities
Belongs to the POMP/UMP1 family. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 51371 Human
- Omim: 613386 Human
- SwissProt: Q9Y244 Human
- Unigene: 268742 Human
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Alternative names
- 2510048O06Rik antibody
- C13orf12 antibody
- Chromosome 13 open reading frame 12 antibody
see all
Images
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Western blot - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)All lanes : Anti-Proteasome maturation protein antibody [EPR10177] (ab170865) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 16 kDa -
![Immunoprecipitation - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)](https://www.abcam.com/ps/products/170/ab170865/Images/ab170865-170749-anti-hspc014-antibody-epr10177-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)Detection of proteasome maturation protein by Western Blot of Immunprecipitate.
HeLa cell lysate immunoprecipitated using ab170865 at 1/10 dilution (Lane 1) or 1X PBS (negative control) (Lane 2) and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
![Flow Cytometry - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)](https://www.abcam.com/ps/products/170/ab170865/Images/ab170865-170748-anti-hspc014-antibody-epr10177-flow-cytometry.jpg)
Flow Cytometry - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)Flow cytometric analysis of permeabilized Jurkat cells labeling proteasome maturation protein with ab170865 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)](https://www.abcam.com/ps/products/170/ab170865/Images/ab170865-170747-anti-hspc014-antibody-epr10177-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)Immunohistochemical analysis of Formalin-fixed,paraffin-embedded Human skin tissue labeling proteasome maturation protein with ab170865 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)](https://www.abcam.com/ps/products/170/ab170865/Images/ab170865-5-benefits-of-recombinant-antibodies.png)
Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)
Protocols
Datasheets and documents
References (2)
ab170865 has been referenced in 2 publications.
- Schneider K et al. Protein Stability Buffers the Cost of Translation Attenuation following eIF2a Phosphorylation. Cell Rep 32:108154 (2020). PubMed: 32937139
- Rousseau A & Bertolotti A An evolutionarily conserved pathway controls proteasome homeostasis. Nature 536:184-9 (2016). WB . PubMed: 27462806
