Recombinant Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Proteasome maturation protein antibody [EPR10177]
See all Proteasome maturation protein primary antibodies -
Description
Rabbit monoclonal [EPR10177] to Proteasome maturation protein -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IP, Flow Cytmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Proteasome maturation protein (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y244 -
Positive control
- HeLa, SW480, HL60 and Jurkat cell lysates; Human skin tissue; Permeabilized Jurkat cells.
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General notes
This product was previously labelled as HSPC014
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10177 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab170865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 16 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum. -
Tissue specificity
Strongly expressed from the basal layer to the granular layer of healthy epidermis, whereas in KLICK patients there is a gradual decrease of expression toward the granular layer. -
Involvement in disease
Defects in POMP are the cause of keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK) [MIM:601952]. KLICK is a keratinizing disorder characterized by ichthyosis, palmoplantar keratoderma with constricting bands around fingers, flexural deformities of fingers and keratotic papules in a linear distribution on the flexural side of large joints. Histological examination of the skin of affected individuals shows hypertrophy and hyperplasia of the spinous, granular and horny epidermal layer. -
Sequence similarities
Belongs to the POMP/UMP1 family. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 51371 Human
- Omim: 613386 Human
- SwissProt: Q9Y244 Human
- Unigene: 268742 Human
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Alternative names
- 2510048O06Rik antibody
- C13orf12 antibody
- Chromosome 13 open reading frame 12 antibody
see all
Images
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![Western blot - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)](https://www.abcam.com/ps/products/170/ab170865/Images/ab170865-170746-anti-hspc014-antibody-epr10177-western-blot.jpg)
Western blot - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)All lanes : Anti-Proteasome maturation protein antibody [EPR10177] (ab170865) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 16 kDa -
![Immunoprecipitation - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)](https://www.abcam.com/ps/products/170/ab170865/Images/ab170865-170749-anti-hspc014-antibody-epr10177-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)Detection of proteasome maturation protein by Western Blot of Immunprecipitate.
HeLa cell lysate immunoprecipitated using ab170865 at 1/10 dilution (Lane 1) or 1X PBS (negative control) (Lane 2) and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
![Flow Cytometry - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)](https://www.abcam.com/ps/products/170/ab170865/Images/ab170865-170748-anti-hspc014-antibody-epr10177-flow-cytometry.jpg)
Flow Cytometry - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)Flow cytometric analysis of permeabilized Jurkat cells labeling proteasome maturation protein with ab170865 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)](https://www.abcam.com/ps/products/170/ab170865/Images/ab170865-170747-anti-hspc014-antibody-epr10177-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome maturation protein antibody [EPR10177] (ab170865)Immunohistochemical analysis of Formalin-fixed,paraffin-embedded Human skin tissue labeling proteasome maturation protein with ab170865 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Rousseau A & Bertolotti A An evolutionarily conserved pathway controls proteasome homeostasis. Nature 536:184-9 (2016). WB . Read more (PubMed: 27462806) »
