Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10177] to Proteasome maturation protein
- Suitable for: WB, IHC-P, IP, Flow Cyt
- Reacts with: Human
Product nameAnti-Proteasome maturation protein antibody [EPR10177]
See all Proteasome maturation protein primary antibodies
DescriptionRabbit monoclonal [EPR10177] to Proteasome maturation protein
Tested applicationsSuitable for: WB, IHC-P, IP, Flow Cytmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Proteasome maturation protein (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y244
- HeLa, SW480, HL60 and Jurkat cell lysates; Human skin tissue; Permeabilized Jurkat cells.
This product was previously labelled as HSPC014
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 16 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMolecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum.
Tissue specificityStrongly expressed from the basal layer to the granular layer of healthy epidermis, whereas in KLICK patients there is a gradual decrease of expression toward the granular layer.
Involvement in diseaseDefects in POMP are the cause of keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK) [MIM:601952]. KLICK is a keratinizing disorder characterized by ichthyosis, palmoplantar keratoderma with constricting bands around fingers, flexural deformities of fingers and keratotic papules in a linear distribution on the flexural side of large joints. Histological examination of the skin of affected individuals shows hypertrophy and hyperplasia of the spinous, granular and horny epidermal layer.
Sequence similaritiesBelongs to the POMP/UMP1 family.
Cellular localizationCytoplasm > cytosol. Nucleus. Microsome membrane.
- Information by UniProt
- 2510048O06Rik antibody
- C13orf12 antibody
- Chromosome 13 open reading frame 12 antibody
All lanes : Anti-Proteasome maturation protein antibody [EPR10177] (ab170865) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 16 kDa
Detection of proteasome maturation protein by Western Blot of Immunprecipitate.
HeLa cell lysate immunoprecipitated using ab170865 at 1/10 dilution (Lane 1) or 1X PBS (negative control) (Lane 2) and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow cytometric analysis of permeabilized Jurkat cells labeling proteasome maturation protein with ab170865 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Immunohistochemical analysis of Formalin-fixed,paraffin-embedded Human skin tissue labeling proteasome maturation protein with ab170865 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab170865 has been referenced in 2 publications.