Key features and details
- Rabbit polyclonal to Proteasome subunit alpha type 6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
Product nameAnti-Proteasome subunit alpha type 6 antibody
See all Proteasome subunit alpha type 6 primary antibodies
DescriptionRabbit polyclonal to Proteasome subunit alpha type 6
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, African green monkey
Predicted to work with: Cow
Recombinant full length protein corresponding to Human Proteasome subunit alpha type 6 aa 1-246.
MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVI VTQKKVPDKLLDSSTVTHLFKITENIGCVMTGMTADSRSQVQRARYEAAN WKYKYGYEIPVDMLCKRIADISQVYTQNAEMRPLGCCMILIGIDEEQGPQ VYKCDPAGYYCGFKATAAGVKQTESTSFLEKKVKKKFDWTFEQTVETAIT CLSTVLSIDFKPSEIEVGVVTVENPKFRILTEAEIDAHLVALAERD
Database link: P60900
- HepG2, HeLa, HL60, COS7, PC12, MCF7, A375, NCI-H460 and Mouse skeletal muscle extracts.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 27 kDa.|
|IHC-P||1/50 - 1/200.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Sequence similaritiesBelongs to the peptidase T1A family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 27 kDa prosomal protein antibody
- IOTA antibody
- Macropain iota chain antibody
All lanes : Anti-Proteasome subunit alpha type 6 antibody (ab185731) at 1/500 dilution
Lane 1 : HepG2 cell line extract
Lane 2 : HeLa cell line extract
Lane 3 : HL60 cell line extract
Lane 4 : COS-7 cell line extract
Lane 5 : PC12 cell line extract
Lane 6 : MCF7 cell line extract
Lane 7 : A375 cell line extract
Lane 8 : NCI-H460 cell line extract
Lane 9 : Mouse skeletal muscle extract
Predicted band size: 27 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach cancer tissue labelling Proteasome subunit alpha type 6 with ab185731 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human rectal cancer tissue labelling Proteasome subunit alpha type 6 with ab185731 at 1/200. Magnification: 400x.
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185731. Blue DAPI for nuclear staining.
ab185731 has not yet been referenced specifically in any publications.