Overview

  • Product name

    Anti-Proteasome subunit alpha type 6 antibody
    See all Proteasome subunit alpha type 6 primary antibodies
  • Description

    Rabbit polyclonal to Proteasome subunit alpha type 6
  • Host species

    Rabbit
  • Specificity

    ab196614 detects endogenous level of total Proteasome subunit alpha type 6 protein.
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Recombinant full length protein within Human Proteasome subunit alpha type 6. The exact sequence is proprietary.
    Database link: P60900

  • Positive control

    • HepG2, HeLa, MCF7, A375, NCL-H460, monkey COS7 and rat PC12 cell lysates; mouse skeletal muscle lysate. Human rectal cancer and stomach cancer tissues. U2OS cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 27 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
  • Sequence similarities

    Belongs to the peptidase T1A family.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 27 kDa prosomal protein antibody
    • IOTA antibody
    • Macropain iota chain antibody
    • Macropain subunit iota antibody
    • MGC22756 antibody
    • MGC2333 antibody
    • MGC23846 antibody
    • Multicatalytic endopeptidase complex iota chain antibody
    • p27K antibody
    • PROS 27 antibody
    • PROS-27 antibody
    • PROS27 antibody
    • Prosomal P27K protein antibody
    • Proteasome (prosome macropain) subunit alpha type 6 antibody
    • Proteasome iota chain antibody
    • Proteasome subunit alpha type 6 antibody
    • Proteasome subunit alpha type-6 antibody
    • Proteasome subunit iota antibody
    • PSA6_HUMAN antibody
    • PSMA 6 antibody
    • PSMA6 antibody
    see all

Images

  • All lanes : Anti-Proteasome subunit alpha type 6 antibody (ab196614) at 1/500 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HL60 cell lysate
    Lane 4 : monkey COS7 cell lysate
    Lane 5 : rat PC12 cell lysate
    Lane 6 : MCF7 cell lysate
    Lane 7 : A375 cell lysate
    Lane 8 : NCL-H460 cell lysate
    Lane 9 : mouse skeletal muscle lysate

    Predicted band size: 27 kDa

  • Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissue labeling Proteasome subunit alpha type 6 using ab196614 at a 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling Proteasome subunit alpha type 6 using ab196614 at a 1/200 dilution.

  • Immunofluorescence analysis of U2OS cells, labeling Proteasome subunit alpha type 6 using ab196614 at a 1/50 dilution. Blue: DAPI for nuclear staining.

References

ab196614 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196614.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up