Key features and details
- Rabbit polyclonal to Proteasome subunit alpha type 6
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Monkey
- Isotype: IgG
Product nameAnti-Proteasome subunit alpha type 6 antibody
See all Proteasome subunit alpha type 6 primary antibodies
DescriptionRabbit polyclonal to Proteasome subunit alpha type 6
Specificityab196614 detects endogenous level of total Proteasome subunit alpha type 6 protein.
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Monkey
Recombinant full length protein within Human Proteasome subunit alpha type 6. The exact sequence is proprietary.
Database link: P60900
- HepG2, HeLa, MCF7, A375, NCL-H460, monkey COS7 and rat PC12 cell lysates; mouse skeletal muscle lysate. Human rectal cancer and stomach cancer tissues. U2OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 27 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Sequence similaritiesBelongs to the peptidase T1A family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 27 kDa prosomal protein antibody
- IOTA antibody
- Macropain iota chain antibody
All lanes : Anti-Proteasome subunit alpha type 6 antibody (ab196614) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : monkey COS7 cell lysate
Lane 5 : rat PC12 cell lysate
Lane 6 : MCF7 cell lysate
Lane 7 : A375 cell lysate
Lane 8 : NCL-H460 cell lysate
Lane 9 : mouse skeletal muscle lysate
Predicted band size: 27 kDa
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissue labeling Proteasome subunit alpha type 6 using ab196614 at a 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling Proteasome subunit alpha type 6 using ab196614 at a 1/200 dilution.
Immunofluorescence analysis of U2OS cells, labeling Proteasome subunit alpha type 6 using ab196614 at a 1/50 dilution. Blue: DAPI for nuclear staining.
ab196614 has not yet been referenced specifically in any publications.