Product nameAnti-Proteasome subunit beta type 2 antibody [EPR8826(2)(B)]
See all Proteasome subunit beta type 2 primary antibodies
DescriptionRabbit monoclonal [EPR8826(2)(B)] to Proteasome subunit beta type 2
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Proteasome subunit beta type 2. The exact sequence is proprietary.
Database link: P49721
- Jurkat, HepG2, HeLa and LoVo cell lysates; Human gastric carcinoma and Human liver tissues; Permeabilized Jurkat cells; Immunoprecipitation pellet from HeLa cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab166628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 23 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
Sequence similaritiesBelongs to the peptidase T1B family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- HC7 I antibody
- Macropain subunit C7 I antibody
- Macropain subunit C7-I antibody
All lanes : Anti-Proteasome subunit beta type 2 antibody [EPR8826(2)(B)] (ab166628) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : LoVo cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Proteasome subunit beta type 2 with ab166628 at a 1/100 dilution.
Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Proteasome subunit beta type 2 with ab166628 at a 1/100 dilution.
Flow cytometric analysis of permeabilized Jurkat cells using ab166628 at a 1/100 dilution (red) or a Rabbit IgG (negative) (green).
Anti-Proteasome subunit beta type 2 antibody [EPR8826(2)(B)] (ab166628) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate at 10 µg
HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
Predicted band size: 23 kDa
This product has been referenced in:
- Cholewa BD et al. Large-scale label-free comparative proteomics analysis of polo-like kinase 1 inhibition via the small-molecule inhibitor BI 6727 (Volasertib) in BRAF(V600E) mutant melanoma cells. J Proteome Res 13:5041-50 (2014). WB . Read more (PubMed: 24884503) »