Recombinant
RabMAb

Anti-Proteasome subunit beta type 2 antibody [EPR8826(2)(B)] (ab166628)

Overview

  • Product name
    Anti-Proteasome subunit beta type 2 antibody [EPR8826(2)(B)]
    See all Proteasome subunit beta type 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR8826(2)(B)] to Proteasome subunit beta type 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Proteasome subunit beta type 2. The exact sequence is proprietary.
    Database link: P49721

  • Positive control
    • Jurkat, HepG2, HeLa and LoVo cell lysates; Human gastric carcinoma and Human liver tissues; Permeabilized Jurkat cells; Immunoprecipitation pellet from HeLa cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR8826(2)(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
  • Sequence similarities
    Belongs to the peptidase T1B family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HC7 I antibody
    • Macropain subunit C7 I antibody
    • Macropain subunit C7-I antibody
    • Multicatalytic endopeptidase complex subunit C7 1 antibody
    • Multicatalytic endopeptidase complex subunit C7 I antibody
    • Multicatalytic endopeptidase complex subunit C7-I antibody
    • Proteasome (prosome, macropain) subunit beta type 2 antibody
    • Proteasome beta 2 subunit antibody
    • Proteasome component C7 I antibody
    • Proteasome component C7-I antibody
    • Proteasome subunit beta type-2 antibody
    • PSB2_HUMAN antibody
    • Psmb2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Proteasome subunit beta type 2 with ab166628 at a 1/100 dilution.
  • Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Proteasome subunit beta type 2 with ab166628 at a 1/100 dilution.
  • Flow cytometric analysis of permeabilized Jurkat cells using ab166628 at a 1/100 dilution (red) or a Rabbit IgG (negative) (green).
  • All lanes : Anti-Proteasome subunit beta type 2 antibody [EPR8826(2)(B)] (ab166628) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : LoVo cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa

  • Anti-Proteasome subunit beta type 2 antibody [EPR8826(2)(B)] (ab166628) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    Predicted band size: 23 kDa

References

This product has been referenced in:
  • Cholewa BD  et al. Large-scale label-free comparative proteomics analysis of polo-like kinase 1 inhibition via the small-molecule inhibitor BI 6727 (Volasertib) in BRAF(V600E) mutant melanoma cells. J Proteome Res 13:5041-50 (2014). WB . Read more (PubMed: 24884503) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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