Recombinant
RabMAb

Anti-Proteasome subunit beta type 2 antibody [EPR8827] (ab137108)

Overview

  • Product name
    Anti-Proteasome subunit beta type 2 antibody [EPR8827]
    See all Proteasome subunit beta type 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR8827] to Proteasome subunit beta type 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, IP, WBmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Proteasome subunit beta type 2 aa 100-200. The exact sequence is proprietary.
    Database link: P49721

  • Positive control
    • HeLa, HepG2, Caco-2 and Jurkat cell lysates. Breast carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR8827
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
Flow Cyt 1/10 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
    • Sequence similarities
      Belongs to the peptidase T1B family.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • HC7 I antibody
      • Macropain subunit C7 I antibody
      • Macropain subunit C7-I antibody
      • Multicatalytic endopeptidase complex subunit C7 1 antibody
      • Multicatalytic endopeptidase complex subunit C7 I antibody
      • Multicatalytic endopeptidase complex subunit C7-I antibody
      • Proteasome (prosome, macropain) subunit beta type 2 antibody
      • Proteasome beta 2 subunit antibody
      • Proteasome component C7 I antibody
      • Proteasome component C7-I antibody
      • Proteasome subunit beta type-2 antibody
      • PSB2_HUMAN antibody
      • Psmb2 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labelling Proteasome subunit beta type 2 with ab137108 at 1/50 dilution.
    • All lanes : Anti-Proteasome subunit beta type 2 antibody [EPR8827] (ab137108) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Caco-2 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 23 kDa

    References

    ab137108 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab137108.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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