Key features and details
- Rabbit polyclonal to Proteasome subunit beta type 2/PSMB2
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Proteasome subunit beta type 2/PSMB2 antibody
See all Proteasome subunit beta type 2/PSMB2 primary antibodies
DescriptionRabbit polyclonal to Proteasome subunit beta type 2/PSMB2
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- Recombinant Human Proteasome subunit beta type 2/PSMB2 protein (ab116169) can be used as a positive control in WB. 293T, HeLa and Jurkat whole cell lysates.
This product was previously labelled as PSMB2, Proteasome subunit beta type 2
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab140426 was affinity purified using an epitope specific to Proteasome subunit beta type 2/PSMB2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab140426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 23 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
Sequence similaritiesBelongs to the peptidase T1B family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- HC7 I antibody
- Macropain subunit C7 I antibody
- Macropain subunit C7-I antibody
All lanes : Anti-Proteasome subunit beta type 2/PSMB2 antibody (ab140426) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 23 kDa
Exposure time: 30 seconds
Detection of Proteasome subunit beta type 2/PSMB2 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab140426 at 6 µg/mg lysate for IP (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry of human ovarian carcinoma tissue using ab140426 at 1/5000.
ab140426 has not yet been referenced specifically in any publications.