Key features and details
- Rabbit polyclonal to Proteasome subunit beta type 2/PSMB2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Proteasome subunit beta type 2/PSMB2 antibody
See all Proteasome subunit beta type 2/PSMB2 primary antibodies
DescriptionRabbit polyclonal to Proteasome subunit beta type 2/PSMB2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Recombinant full length protein corresponding to Human Proteasome subunit beta type 2/PSMB2 aa 1-201.
MEYLIGIQGPDYVLVASDRVAASNIVQMKDDHDKMFKMSEKILLLCVGEA GDTVQFAEYIQKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHV NLLLAGYDEHEGPALYYMDYLAALAKAPFAAHGYGAFLTLSILDRYYTPT ISRERAVELLRKCLEELQKRFILNLPTFSVRIIDKNGIHDLDNISFPKQG S
Database link: P49721
- WB: EC109 and HEK-293T whole cell lysate. IHC-P: Mouse kidney tissue. ICC/IF: PC-3 cells.
This product was previously labelled as PSMB2, Proteasome subunit beta type 2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 23 kDa.|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
Sequence similaritiesBelongs to the peptidase T1B family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- HC7 I antibody
- Macropain subunit C7 I antibody
- Macropain subunit C7-I antibody
PC-3 (human prostate adenocarcinoma cell line) cells stained for Proteasome subunit beta type 2/PSMB2 (green) using ab236752 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
Paraffin-embedded mouse kidney tissue stained for Proteasome subunit beta type 2/PSMB2 using ab236752 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-Proteasome subunit beta type 2/PSMB2 antibody (ab236752) at 1/500 dilution
Lane 1 : EC109 whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size: 23 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236752 has not yet been referenced specifically in any publications.