Recombinant
RabMAb

Recombinant Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)

Reviews (5) Submit a question References (1)

Overview

  • Product name

    Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)]
    See all Proteasome subunit beta type 2/PSMB2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8826(2)(B)] to Proteasome subunit beta type 2/PSMB2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Proteasome subunit beta type 2/PSMB2. The exact sequence is proprietary.
    Database link: P49721

  • Positive control

    • Jurkat, HepG2, HeLa and LoVo cell lysates; Human gastric carcinoma and Human liver tissues; Permeabilized Jurkat cells; Immunoprecipitation pellet from HeLa cell lysate.

  • General notes

     This product was previously labelled as PSMB2, Proteasome subunit beta type 2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8826(2)(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.

  • Sequence similarities

    Belongs to the peptidase T1B family.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • HC7 I antibody
    • Macropain subunit C7 I antibody
    • Macropain subunit C7-I antibody
    • Multicatalytic endopeptidase complex subunit C7 1 antibody
    • Multicatalytic endopeptidase complex subunit C7 I antibody
    • Multicatalytic endopeptidase complex subunit C7-I antibody
    • Proteasome (prosome, macropain) subunit beta type 2 antibody
    • Proteasome beta 2 subunit antibody
    • Proteasome component C7 I antibody
    • Proteasome component C7-I antibody
    • Proteasome subunit beta type-2 antibody
    • PSB2_HUMAN antibody
    • Psmb2 antibody
    see all

Images

  • Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    All lanes : Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : LoVo cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Proteasome subunit beta type 2/PSMB2 with ab166628 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)

    Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Proteasome subunit beta type 2/PSMB2 with ab166628 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Flow Cytometry - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Flow cytometric analysis of permeabilized Jurkat cells using ab166628 at a 1/100 dilution (red) or a Rabbit IgG (negative) (green).
  • Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    Predicted band size: 23 kDa

References

This product has been referenced in:

  • Cholewa BD  et al. Large-scale label-free comparative proteomics analysis of polo-like kinase 1 inhibition via the small-molecule inhibitor BI 6727 (Volasertib) in BRAF(V600E) mutant melanoma cells. J Proteome Res 13:5041-50 (2014). WB . Read more (PubMed: 24884503) »
See 1 Publication for this product

Publishing research using ab166628? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Immunoprecipitation abreview for Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)]

 Average
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (MEF cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
MEF cells
Read More

Abcam user community

Verified customer

Submitted Nov 08 2019

Immunoprecipitation abreview for Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)]

 Excellent
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
293T cells
Read More

Abcam user community

Verified customer

Submitted Nov 07 2019

Western blot abreview for Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
MEF cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Feb 12 2019

Western blot abreview for Anti-Proteasome subunit beta type 2 antibody [EPR8826(2)(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
293T cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 24 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Proteasome subunit beta type 2 antibody [EPR8826(2)(B)]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Read More

Dr. Kirk Mcmanus

Verified customer

Submitted Jan 14 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com