Product nameAnti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827]
See all Proteasome subunit beta type 2/PSMB2 primary antibodies
DescriptionRabbit monoclonal [EPR8827] to Proteasome subunit beta type 2/PSMB2
Tested applicationsSuitable for: IHC-P, Flow Cyt, IP, WBmore details
Unsuitable for: ICC
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Proteasome subunit beta type 2/PSMB2 aa 100-200. The exact sequence is proprietary.
Database link: P49721
- HeLa, HepG2, Caco-2 and Jurkat cell lysates. Breast carcinoma tissue.
This product was previously labelled as PSMB2, Proteasome subunit beta type 2
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- HeLa nuclear extract lysate (ab14655)
- HepG2 cytoplasmic extract lysate (ab14659)
- HepG2 nuclear extract lysate (ab14660)
- Jurkat cytoplasmic extract lysate (ab14842)
- Jurkat nuclear extract lysate (ab14844)
- HeLa whole cell lysate (ab29545)
- Caco 2 whole cell lysate (ab3950)
- Jurkat whole cell lysate (ab7899)
- HepG2 whole cell lysate (ab7900)
Our Abpromise guarantee covers the use of ab137108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 23 kDa.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
Sequence similaritiesBelongs to the peptidase T1B family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- HC7 I antibody
- Macropain subunit C7 I antibody
- Macropain subunit C7-I antibody
Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labelling Proteasome subunit beta type 2/PSMB2 with ab137108 at 1/50 dilution.
All lanes : Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 23 kDa
ab137108 has not yet been referenced specifically in any publications.