Recombinant
RabMAb

Recombinant Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108)

Overview

  • Product name

    Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827]
    See all Proteasome subunit beta type 2/PSMB2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8827] to Proteasome subunit beta type 2/PSMB2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, IP, WBmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Proteasome subunit beta type 2/PSMB2 aa 100-200. The exact sequence is proprietary.
    Database link: P49721

  • Positive control

    • HeLa, HepG2, Caco-2 and Jurkat cell lysates. Breast carcinoma tissue.
  • General notes

     

     

     This product was previously labelled as PSMB2, Proteasome subunit beta type 2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8827
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
Flow Cyt 1/10 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
    • Sequence similarities

      Belongs to the peptidase T1B family.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • HC7 I antibody
      • Macropain subunit C7 I antibody
      • Macropain subunit C7-I antibody
      • Multicatalytic endopeptidase complex subunit C7 1 antibody
      • Multicatalytic endopeptidase complex subunit C7 I antibody
      • Multicatalytic endopeptidase complex subunit C7-I antibody
      • Proteasome (prosome, macropain) subunit beta type 2 antibody
      • Proteasome beta 2 subunit antibody
      • Proteasome component C7 I antibody
      • Proteasome component C7-I antibody
      • Proteasome subunit beta type-2 antibody
      • PSB2_HUMAN antibody
      • Psmb2 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labelling Proteasome subunit beta type 2/PSMB2 with ab137108 at 1/50 dilution.

    • All lanes : Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Caco-2 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 23 kDa

    References

    ab137108 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - whole cell (MEF cells)
    Total protein in input
    500 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    MEF cells

    Abcam user community

    Verified customer

    Submitted Jan 24 2019

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    50 µg
    Specification
    MEF cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 03 2018

    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (293T cells)
    Total protein in input
    500 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    293T cells

    Abcam user community

    Verified customer

    Submitted Nov 30 2018

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    50 µg
    Specification
    293T cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Nov 26 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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