Recombinant Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108)](https://www.abcam.com/ps/products/137/ab137108/Images/ab137108-151714-anti-proteasome-subunit-beta-type-2-antibody-epr8827-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8827] to Proteasome subunit beta type 2/PSMB2
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827]
See all Proteasome subunit beta type 2/PSMB2 primary antibodies -
Description
Rabbit monoclonal [EPR8827] to Proteasome subunit beta type 2/PSMB2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Proteasome subunit beta type 2/PSMB2 aa 100-200. The exact sequence is proprietary.
Database link: P49721 -
Positive control
- HeLa, HepG2, Caco-2 and Jurkat cell lysates. Breast carcinoma tissue.
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General notes
This product was previously labelled as PSMB2, Proteasome subunit beta type 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8827 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
|
| Notes |
|---|
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity. -
Sequence similarities
Belongs to the peptidase T1B family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5690 Human
- Entrez Gene: 26445 Mouse
- Entrez Gene: 29675 Rat
- Omim: 602175 Human
- SwissProt: P49721 Human
- SwissProt: Q9R1P3 Mouse
- SwissProt: P40307 Rat
- Unigene: 471441 Human
see all -
Alternative names
- HC7 I antibody
- Macropain subunit C7 I antibody
- Macropain subunit C7-I antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108)
Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labelling Proteasome subunit beta type 2/PSMB2 with ab137108 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108)](https://www.abcam.com/ps/products/137/ab137108/Images/ab137108-151715-anti-proteasome-subunit-beta-type-2-antibody-epr8827-western-blot.jpg)
Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108)All lanes : Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 23 kDa -
![Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108)](https://www.abcam.com/ps/products/137/ab137108/Images/ab137108-3-benefits-of-recombinant-antibodies.png)
Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8827] (ab137108)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137108 has not yet been referenced specifically in any publications.