Key features and details
- Mouse monoclonal [MCP165] to Proteasome subunit beta type 2/PSMB2
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165]
See all Proteasome subunit beta type 2/PSMB2 primary antibodies
DescriptionMouse monoclonal [MCP165] to Proteasome subunit beta type 2/PSMB2
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Full length protein corresponding to Human Proteasome subunit beta type 2/PSMB2.
Database link: P49721
- WB: HeLa cell lysate; human ciliary body lysate. IHC-P: Human lymphoid tissue. Flow cyt: HeLa cells.
This product was previously labelled as PSMB2, Proteasome subunit beta type 2
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.00
Preservative: 0.032% Sodium azide
Constituents: 0.316% Tris HCl, 0.58% Sodium chloride
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab22650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
Sequence similaritiesBelongs to the peptidase T1B family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- HC7 I antibody
- Macropain subunit C7 I antibody
- Macropain subunit C7-I antibody
Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (ab22650) at 1/1000 dilution + HeLa cell lysate
Predicted band size: 23 kDa
Lane 1 : Protein Marker IV
Lanes 2-7 : Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (ab22650) at 1/1000 dilution
Lane 1 : Protein Marker IV
Lanes 2 & 4 & 6 : Protein extract from ciliary body of diseased human donor at 2 µg
Lanes 3 & 5 & 7 : Protein extract from ciliary body of normal human donor at 2 µg
Lanes 2-7 : HRP conjugated goat anti mouse antibody
Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
IHC image of ab22650 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22650, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Overlay histogram showing HeLa cells stained with ab22650 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22650, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab22650 has been referenced in 4 publications.
- Leite NC et al. Protein malnutrition potentiates the amplifying pathway of insulin secretion in adult obese mice. Sci Rep 6:33464 (2016). WB ; Mouse . PubMed: 27633083
- Ren H et al. Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice. Sci Rep 5:15730 (2015). PubMed: 26508670
- Arellano-Garcia ME et al. Interferon-? induces immunoproteasomes and the presentation of MHC I-associated peptides on human salivary gland cells. PLoS One 9:e102878 (2014). WB ; Human . PubMed: 25102056
- Durgeau A et al. Different expression levels of the TAP peptide transporter lead to recognition of different antigenic peptides by tumor-specific CTL. J Immunol 187:5532-9 (2011). WB ; Human . PubMed: 22025554