Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (ab22650)

Overview

  • Product name

    Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165]
    See all Proteasome subunit beta type 2/PSMB2 primary antibodies
  • Description

    Mouse monoclonal [MCP165] to Proteasome subunit beta type 2/PSMB2
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length protein corresponding to Human Proteasome subunit beta type 2/PSMB2.
    Database link: P49721

  • Positive control

    • WB: HeLa cell lysate; human ciliary body lysate. IHC-P: Human lymphoid tissue. Flow cyt: HeLa cells.
  • General notes

     This product was previously labelled as PSMB2, Proteasome subunit beta type 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab22650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity.
    • Sequence similarities

      Belongs to the peptidase T1B family.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • HC7 I antibody
      • Macropain subunit C7 I antibody
      • Macropain subunit C7-I antibody
      • Multicatalytic endopeptidase complex subunit C7 1 antibody
      • Multicatalytic endopeptidase complex subunit C7 I antibody
      • Multicatalytic endopeptidase complex subunit C7-I antibody
      • Proteasome (prosome, macropain) subunit beta type 2 antibody
      • Proteasome beta 2 subunit antibody
      • Proteasome component C7 I antibody
      • Proteasome component C7-I antibody
      • Proteasome subunit beta type-2 antibody
      • PSB2_HUMAN antibody
      • Psmb2 antibody
      see all

    Images

    • Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (ab22650) at 1/1000 dilution + HeLa cell lysate

      Predicted band size: 23 kDa

    • Lane 1 : Protein Marker IV
      Lanes 2-7 : Anti-Proteasome subunit beta type 2/PSMB2 antibody [MCP165] (ab22650) at 1/1000 dilution

      Lane 1 : Protein Marker IV
      Lanes 2 & 4 & 6 : Protein extract from ciliary body of diseased human donor at 2 µg
      Lanes 3 & 5 & 7 : Protein extract from ciliary body of normal human donor at 2 µg

      Secondary
      Lanes 2-7 : HRP conjugated goat anti mouse antibody

      Predicted band size: 23 kDa
      Observed band size: 27 kDa
      why is the actual band size different from the predicted?

      See Abreview

    • IHC image of ab22650 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22650, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Overlay histogram showing HeLa cells stained with ab22650 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22650, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References

    This product has been referenced in:

    • Leite NC  et al. Protein malnutrition potentiates the amplifying pathway of insulin secretion in adult obese mice. Sci Rep 6:33464 (2016). WB ; Mouse . Read more (PubMed: 27633083) »
    • Ren H  et al. Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice. Sci Rep 5:15730 (2015). Read more (PubMed: 26508670) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer


    I can confirm that this particular antibody is sourced externally from a collaborator. Unfortunately we are not able to release the immunogen sequence as they consider this proprietary information.

    They have confirmed that this antibody recognizes the β2 subunit of the 20S proteasome and the immunogen is dinitrophenylated proteasomes.

    We aim to provide as much information as we can to our customers, so I am sorry that this has not been possible on this occasion.

    Read More
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (ciliary body)
    Loading amount
    2 µg
    Specification
    ciliary body
    Blocking step
    BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%

    Dr. Matthias Zenkel

    Verified customer

    Submitted May 04 2007

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