Overview

  • Product name
    Anti-Proteasome subunit beta type 4 antibody
    See all Proteasome subunit beta type 4 primary antibodies
  • Description
    Goat polyclonal to Proteasome subunit beta type 4
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Hamster, Cow, Dog, Pig, Monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human Proteasome subunit beta type 4 aa 233-243 (internal sequence) (Cysteine residue).
    Sequence:

    QIATVTEKGVE


    Database link: P28070

  • Positive control
    • Human skin tissue; HEK293, HeLa, Jurkat and K562 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 29 kDa).
IHC-P Use a concentration of 3.75 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • ab166792 staining Proteasome subunit beta type 4 in Human U2OS Osteo sarcome cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in 1% BSA + 1% donkey serum in 1x PBS +0.5%T) for 8 hours at 4°C. An Alexa Fluor® 647-conjugated Donkey anti-goat IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-Proteasome subunit beta type 4 antibody (ab166792) at 0.3 µg/ml + HEK293 cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?



    Primary incubation: 1 hour.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling Proteasome subunit beta type 4 with ab166792 at 3.75 µg/ml.

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Sample
Human Cell (U2OS Osteo sarcome)
Specification
U2OS Osteo sarcome
Permeabilization
Yes - tritonX100
Fixative
Formaldehyde

Mr. Gabriel Ortega

Verified customer

Submitted Sep 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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