Product nameProtein A (20nm Gold)
Protein A (20nm Gold) (ab270601) is manufactured using InnovaCoat® technology, a unique coated nanoparticle that has an avidly bound protective surface coat which can withstand the most extreme conditions (e.g. 2.5M NaOH at 70°C for >1 hour).
This product is manufactured by Expedeon, an Abcam company. It was previously called 20nm InnovaCoat® GOLD (10 OD) Protein A Conjugate. Expedeon product code 224-0200 is the same as the 200 µl size of this product and code 224-1000 is the same as the 1 mL size.
The Protein A (from S. Aureus) is irreversibly attached to the surface coat via a covalent bond. This irreversible attachment means that the Protein A cannot desorb. Protein A desorption is a major problem with products where the Protein A is only passively adsorbed to the metal.
ab270601 is shipped in a buffer composed of TBS containing 0.1% detergent. If you wish to exchange into a specific buffer for your assay or test, centrifuge the conjugate in a microfuge at 9,000 x g for 20 minutes. Carefully remove the supernatant and add your preferred buffer. It is important to avoid substances that have a very high affinity for gold (e.g. thiols).
The maximum absorbance for the 20nm IC-GOLD Protein A is at 528nm.
To determine the effective concentration of the conjugate obtained we advise to measure the Absmax of light using an UV-vis spectrophotometer after diluting your sample to an appropriate range for your piece of equipment (e.g. if a 40nm gold conjugate is at 10 OD and is diluted 1:10 the Abs530nm for a 1 cm light path is expected to be around 1 AU).
Before using InnovaCoat GOLD Protein A 20nm and 40nm to detect a specific antibody we recommend that you check the affinity of Protein A for that antibody and subtype (see table below). Rabbit and human IgGs, for example, are good candidates because they bind to Protein A with high affinity.
Species Ig Binding Strength Rabbit IgG High Human IgG High Pig IgG High Mouse IgG1 Low/Medium Mouse IgG2a High Mouse IgG2b High Mouse IgG3 Low/Medium Goat IgG Low Sheep IgG Low Rat IgG Low
InnovaCoat GOLD Protein A 20nm and 40nm is suitable as a detector tool in electron and light microscopy, immunoblotting, dot-blot and lateral flow assays applications. As InnovaCoat GOLD Protein A affinity for antibodies can vary, a titration of the specific antibody used is recommended in order to obtain the optimal performance. We also recommend performing the incubation of InnovaCoat GOLD Protein A 20nm and 40nm with the antibody in a buffer that has a pH close to 8, for at least 10-15 minutes. After this stage do not centrifuge the gold complex.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituents: Tris buffered saline, 0.1% Tween
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab270601 has not yet been referenced specifically in any publications.