Product nameProtein A Agarose (High Affinity)
Sample typeCell culture supernatant, Serum, Cell culture media, Ascites Fluid
Very high binding capacity (>35 mg IgG/mL). Minimal leaching of ligand. Suitable for column or batch purification of IgG, immunoprecipitation & ChIP (ab193255).
Supplied as 50% slurry in 20% Ethanol.
High binding capacity = Binding of IgG ≥ 36 mg human or rabbit IgG/mL Protein A Agarose.
Minimal leaching of the ligand
Flow Rate Tested* = 2.89 mL/min.
*Test conditions: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).
Maximum Flow Rate** = 1800 cm/hr; minimum leaching of recombinant Protein A.
**NOTE = the highest flow that beads withstand for 1 minute, without collapsing and the pressure reaching 1 MPa.
Usage = Reusable for up to 10 times without significant loss of binding capacity.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
Abcam's Protein A Agarose (High Affinity) beads are specially prepared for high IgG binding by covalently coupling recombinant Protein A to 6% cross-linked agarose beads, the most popular resin for protein affinity purification methods. Protein A is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A compared to standard Protein A agarose beads. The IgG binding capacity of Protein A Agarose (High Affinity) is ≥ 36 mg human or rabbit IgG per mL of wet beads. The Protein A Agarose (High Affinity) beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.
Tested applicationsSuitable for: IP, Purificationmore details
Our Abpromise guarantee covers the use of ab193255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
|Purification||Use at an assay dependent concentration.
Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab193255 has been referenced in 1 publication.
- Wu J et al. PHA-4/FoxA senses nucleolar stress to regulate lipid accumulation in Caenorhabditis elegans. Nat Commun 9:1195 (2018). PubMed: 29567958