Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab19483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.
ab19483 can work in direct ELISA, but not indirect ELISA (because any secondary antibody would bind to protein A directly as well as the primary antibody, thus leading to high background – due to the nature of protein A). ab18596 as detection antibody for sELISA.
|WB||1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
50 kDa band is due to cleavage of the signal peptide and propeptide
RelevanceProtein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Cellular localizationSecreted; cell wall.
- gG-binding protein A antibody
- Immunoglobulin G binding protein A antibody
- SPA antibody
- Staphylococcal protein A antibody
ab19483 has not yet been referenced specifically in any publications.