Overview

  • Product name
  • Description
    Chicken polyclonal to Protein A
  • Host species
    Chicken
  • Tested applications
    Suitable for: Sandwich ELISA, WBmore details
  • Immunogen

    Recombinant full length protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

ab19483 can work in direct ELISA, but not indirect ELISA (because any secondary antibody would bind to protein A directly as well as the primary antibody, thus leading to high background – due to the nature of protein A). ab18596 as detection antibody for sELISA.

WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).

50 kDa band is due to cleavage of the signal peptide and propeptide

Target

  • Relevance
    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localization
    Secreted; cell wall.
  • Alternative names
    • gG-binding protein A antibody
    • Immunoglobulin G binding protein A antibody
    • SPA antibody
    • Staphylococcal protein A antibody

References

ab19483 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Answer

We do not have specific use by dates for our antibodies. If stored correctly, antibodies remain suitable for use for months and even for years. Please read our instructions on how to store antibodies on our website (https://www.abcam.com/index.html?pageconfig=popular_protocols). However, we do suggest the following approximate use by dates for all products based from timne of delivery: room temperature: 1 week + 4°C: 1 year - 20°C: 5 years - 80°C: 10 years These products are covered by our Abpromise guarantee fro a period of one year. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within twelve months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue.

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Answer

Thank you for contacting us.

Yes, you are right. The number after the dash signifies when the lot was aliquoted. If the number directly after the "GR" is the same then it is the same lot.

For ab19483, chicken polyclonal to Protein A, we have 16 vials of lot GR58389-4 in our US office ready to ship and an additional 30 units of lots GR58389-3 and GR58389-4 in our UK office.

For ab18596, chicken polyclonal to Protein A (HRP), we have 1 vial of GR74278-4 in our UK office which would take a few extra days to ship to you. We have two new lots in for that antibody, but only 1 vial left of the lot you requested.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

We currently have x vials of ab19483, lot GR58389 worldwide (x in UK office, x in US office, x in Japan office.)

For ab18596, we only have 1 vial of lot GR74278 in stock, however we do hold 8 other vials of different stocks of this antibody in the UK.

The way we number our lots is indeed the way you mentioned. The first part of the lot number e.g GR12345 corresponds to one particular lot. The last number e.g GR12345-X corresponds to the a particular date where we receieved the antibody from our labs. This means that lots GR12345-1 and GR12345-2 of a particularly antibody originate from the same stock but were delivered to our stock rooms at different days therefore these should be identical.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry and your interest in our products.

I have checked the stock viability and can confirm the followings:

- ab19483 - several vials from the same bleed and purification;

- ab18596 - only one vial left from the batch required

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for your inquiry.

I attached the ELISA testing data of ab19483 to this email for your information and wish you good lcuk with your research.

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Question
Answer

Thank you very much for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

I have attached the ELISA data and graph for ab19483.

I hope that this information will be useful, but if there is anything else that you need from us please let me know and I'll be happy to help.

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Question
Answer

Thank you very much for your call last week and for your patience while I've been in contact with the lab regarding your enquiry.

My colleagues are gathering this data and we will send it to you as soon as possible. I apologize for the delay, but please let me know if there is anything else that we can do for you in the meantime.

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Answer

Thank you for contacting Abcam regarding ab18598 and ab19483.


Attached please find the CoC documents you requested.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Answer

Thank you for contacting us.

The conjugated antibodies ab18596 and ab18598 are prepared from the unconjugated ab19483. The formulations differ (for instance, there is no azide in the HRP conjugate buffer) but the antibody is the same for all three.

I fixed a mistake on the ab19483 datasheet regarding the purity, which was inconsistent with the other two datasheets. It is immunogen-affinity-purified.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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