• Product name
  • Description
    Chicken polyclonal to Protein A
  • Host species
  • Tested applications
    Suitable for: Sandwich ELISA, WBmore details
  • Immunogen

    Recombinant full length protein.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab19483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

ab19483 can work in direct ELISA, but not indirect ELISA (because any secondary antibody would bind to protein A directly as well as the primary antibody, thus leading to high background – due to the nature of protein A). ab18596 as detection antibody for sELISA.

WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).

50 kDa band is due to cleavage of the signal peptide and propeptide


  • Relevance
    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localization
    Secreted; cell wall.
  • Alternative names
    • gG-binding protein A antibody
    • Immunoglobulin G binding protein A antibody
    • SPA antibody
    • Staphylococcal protein A antibody


ab19483 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

We do not have specific use by dates for our antibodies. If stored correctly, antibodies remain suitable for use for months and even for years. Please read our instructions on how to store antibodies on our website (https://www.abcam.com/index.html?pag...

Read More

Thank you for contacting us.

Yes, you are right. The number after the dash signifies when the lot was aliquoted. If the number directly after the "GR" is the same then it is the same lot.

For ab19483, chicken polyclonal t...

Read More

Thank you for contacting us.

We currently have x vials of ab19483, lot GR58389 worldwide (x in UK office, x in US office, x in Japan office.)

For ab18596, we only have 1 vial of lot GR74278 in stock, however we do hold 8 other vial...

Read More

Thank you for your enquiry and your interest in our products.

I have checked the stock viability and can confirm the followings:

- ab19483 - several vials from the same bleed and purification;

- ab18596 - only one vial lef...

Read More

Thank you for your inquiry.

I attached the ELISA testing data of ab19483 to this email for your information and wish you good lcuk with your research.

Thank you very much for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

I have attached the ELISA data and graph for ab19483.

I hope that this information will be useful, but...

Read More

Thank you very much for your call last week and for your patience while I've been in contact with the lab regarding your enquiry.

My colleagues are gathering this data and we will send it to you as soon as possible. I apologize for the delay,...

Read More

Thank you for contacting us. I apologize for being late in getting back to you.

I can confirm that both antibodies ab18596 and ab19483 have similar IgY isotype.

I hope this information is helpful to you. Please do not hesitate to cont...

Read More

Thank you for contacting Abcam regarding ab18598 and ab19483.

Attached please find the CoC documents you requested.

I hope this information is helpful. Please do not hesitate to contact us if you have any additional ques...

Read More

Thank you for contacting us.

The conjugated antibodies ab18596 and ab18598 are prepared from the unconjugated ab19483. The formulations differ (for instance, there is no azide in the HRP conjugate buffer) but the antibody is the same for all...

Read More

1-10 of 11 Abreviews or Q&A


Sign up