Overview

  • Product name

    Anti-Protein A antibody (Biotin)
    See all Protein A primary antibodies
  • Description

    Chicken polyclonal to Protein A (Biotin)
  • Host species

    Chicken
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Immunogen

    Recombinant full length protein corresponding to Staphylococcus aureus Protein A.
    Database link: P02976

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    After multiple immunizations in Freund’s adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein A agarose and conjugated with biotin.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab18598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
ELISA 1/10000 - 1/50000.

Target

  • Relevance

    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localization

    Secreted; cell wall.
  • Alternative names

    • gG-binding protein A antibody
    • Immunoglobulin G binding protein A antibody
    • SPA antibody
    • Staphylococcal protein A antibody

References

This product has been referenced in:

  • Abuaita BH  et al. Mitochondria-Derived Vesicles Deliver Antimicrobial Reactive Oxygen Species to Control Phagosome-Localized Staphylococcus aureus. Cell Host Microbe 24:625-636.e5 (2018). Read more (PubMed: 30449314) »
  • Gilbert FB  et al. Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system. Vet Res 44:40 (2013). WB ; Staphylococcus aureus . Read more (PubMed: 23758654) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting Abcam regarding ab18598 and ab19483.


Attached please find the CoC documents you requested.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Answer

Thank you for contacting us.

The conjugated antibodies ab18596 and ab18598 are prepared from the unconjugated ab19483. The formulations differ (for instance, there is no azide in the HRP conjugate buffer) but the antibody is the same for all three.

I fixed a mistake on the ab19483 datasheet regarding the purity, which was inconsistent with the other two datasheets. It is immunogen-affinity-purified.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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