Key features and details
- Chicken polyclonal to Protein A (Biotin)
- Suitable for: WB, ELISA
- Conjugation: Biotin
- Isotype: IgY
Product nameAnti-Protein A antibody (Biotin)
See all Protein A primary antibodies
DescriptionChicken polyclonal to Protein A (Biotin)
Tested applicationsSuitable for: WB, ELISAmore details
Recombinant full length protein corresponding to Staphylococcus aureus Protein A.
Database link: P02976
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAfter multiple immunizations in Freund’s adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein A agarose and conjugated with biotin.
Our Abpromise guarantee covers the use of ab18598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|ELISA||1/10000 - 1/50000.|
RelevanceProtein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Cellular localizationSecreted; cell wall.
- gG-binding protein A antibody
- Immunoglobulin G binding protein A antibody
- SPA antibody
- Staphylococcal protein A antibody
ab18598 has been referenced in 2 publications.
- Abuaita BH et al. Mitochondria-Derived Vesicles Deliver Antimicrobial Reactive Oxygen Species to Control Phagosome-Localized Staphylococcus aureus. Cell Host Microbe 24:625-636.e5 (2018). PubMed: 30449314
- Gilbert FB et al. Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system. Vet Res 44:40 (2013). WB ; Staphylococcus aureus . PubMed: 23758654