Overview

  • Product name
    Anti-Protein A antibody (HRP)
    See all Protein A primary antibodies
  • Description
    Chicken polyclonal to Protein A (HRP)
  • Host species
    Chicken
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Immunogen

    Recombinant full length protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.00
    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab18596 was immunoaffinity purified off protein A agarose and conjugated with horseradish peroxidase (HRP).
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
ELISA 1/5000 - 1/20000.

Target

  • Relevance
    Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • Cellular localization
    Secreted; cell wall.
  • Alternative names
    • gG-binding protein A antibody
    • Immunoglobulin G binding protein A antibody
    • SPA antibody
    • Staphylococcal protein A antibody

Images

References

ab18596 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Answer

It appears that you are asking if a capture(sandwich) ELISA was performed.

We do not run a capture test on the the conjugate, only a direct ELISA to determine the functionality of the conjugation.

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Answer

We do not have specific use by dates for our antibodies. If stored correctly, antibodies remain suitable for use for months and even for years. Please read our instructions on how to store antibodies on our website (https://www.abcam.com/index.html?pageconfig=popular_protocols). However, we do suggest the following approximate use by dates for all products based from timne of delivery: room temperature: 1 week + 4°C: 1 year - 20°C: 5 years - 80°C: 10 years These products are covered by our Abpromise guarantee fro a period of one year. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within twelve months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue.

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Answer

Thank you for contacting us.

Worldwide, we have 16 vials of this antibody in stock. We do not have more as it is not a bestseller, but sells quite solid. This product is not a niche product either, therefore, we constantly have have a certain threshold in stock.

In case you want to buy a higher quantity of a certain lot, please let our customer service team, so we can order it with the lab for you.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

Yes, you are right. The number after the dash signifies when the lot was aliquoted. If the number directly after the "GR" is the same then it is the same lot.

For ab19483, chicken polyclonal to Protein A, we have 16 vials of lot GR58389-4 in our US office ready to ship and an additional 30 units of lots GR58389-3 and GR58389-4 in our UK office.

For ab18596, chicken polyclonal to Protein A (HRP), we have 1 vial of GR74278-4 in our UK office which would take a few extra days to ship to you. We have two new lots in for that antibody, but only 1 vial left of the lot you requested.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

We currently have x vials of ab19483, lot GR58389 worldwide (x in UK office, x in US office, x in Japan office.)

For ab18596, we only have 1 vial of lot GR74278 in stock, however we do hold 8 other vials of different stocks of this antibody in the UK.

The way we number our lots is indeed the way you mentioned. The first part of the lot number e.g GR12345 corresponds to one particular lot. The last number e.g GR12345-X corresponds to the a particular date where we receieved the antibody from our labs. This means that lots GR12345-1 and GR12345-2 of a particularly antibody originate from the same stock but were delivered to our stock rooms at different days therefore these should be identical.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry and your interest in our products.

I have checked the stock viability and can confirm the followings:

- ab19483 - several vials from the same bleed and purification;

- ab18596 - only one vial left from the batch required

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for confirming this information and for your help and cooperation with this case.

As a special exception as the customer has used so many vials succesfully, we can extend the guarantee period in this case and Iasked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

Credit ID: 21177

As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

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Answer

Thank you for your reply.

I appreciate your cooperation and understand your concerns. It is regrettable the results from lot 793467have not been assatisfactory for the customer. I can confirm that ab18596 and ab18596-1 are the same antibody. The -1 just relates to the size.

(I assume there was a typing error in your email, ab18596 and ab18569-1 are not the same antibody).

With regards to the COA request, some of our collaborators are not certified ISO9001 and so we are not able to provide a certificate of analysis for these particular products. As the identity of our collaborators is confidential, we provide a certificate of compliance (COC)for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product. I am going to request the QC testing data for these 2 lots from our source, I assume this is what your customer would like to see for their reassurance?

In order to help with our investigation of this case before deciding how to proceed, I would appreciate if you are able to provide more details. This information will also be helpful to our quality monitoring.

1. Please provide the order numbers and date of purchase.

2. I would appreciate if you are able to provide the data from each of these lots in order to help us assess the results.

Thank you for your time. I look forward to hearing from you with the requested information and hope we can resolve this case as soon as possible.

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Answer

As this is a polyclonal antibody, some batch variability could be expected. It is possible there could also be variabilitywith the detection kit or secondary antibodyin this case.

Could you confirm if the customer is satisfied with the results with the longer incubation? We would be pleased to investigate this further if the results are not as the customer is expecting.

I would like to reassure you that this antibody is tested and covered by our Abpromise guarantee for ELISA. In the event that a product is not functioning in the tested applications and species cited on the product data sheet, we will be pleased to provide a credit note, free of charge replacement or refund.

www.abcam.com/abpromise

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1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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