Overview

  • Product name

    Protein A ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low 127.9pg/ml 5.2%
    Medium 364.3pg/ml 6.4%
    High 727.8pg/ml 5.5%
    Inter-assay
    Sample n Mean SD CV%
    Low 103.6pg/ml 13.4%
    Medium 209.7pg/ml 8.1%
    High 378.3pg/ml 8%
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.35 pg/ml
  • Range

    15.63 pg/ml - 1000 pg/ml
  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: a wide range of other species
  • Product overview

    Abcam’s Protein A in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of natural and recombinant Protein A in neutralized buffers.


    A chicken IgY antibody specific to Protein A has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A Protein A polyclonal antibody is then added, incubated and washed. An HRP conjugated antibody is then added incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound

  • Notes

    Staphylococcus aureus Protein A is a 42 kDa cell wall constituent that is characterized by its binding affinity to the Fc portion of some immunoglobulins, especially the IgG class. The IgG binding domain (domain B) consists of three anti-parallel alpha-helices, the third of which is disrupted when the protein is complexed with Fc. Protein A is commonly used to purify antisera and in commonly used immunodetection and visualization techniques. Protein A participates in a number of protective biological functions including anti-tumor, toxic and carcinogenic activities, thus necessitating the removal of potential Protein A contaminants from antibody preparations for therapeutic use.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 100ml
    Assay Buffer 13 1 x 55ml
    Chicken anti-Protein A Microplate (12 x 8 wells) 1 unit
    Plate Sealer 2 units
    Protein A Antibody 1 x 10ml
    Protein A HRP-Streptavidin Conjugate 1 x 10ml
    Protein A Standard 1 vial
    Stop Solution 2 1 x 10ml
    TMB Substrate 1 x 10ml
  • Research areas

  • Cellular localization

    Secreted
  • Alternative names

    • Protein A
    • SpA

Applications

Our Abpromise guarantee covers the use of ab133036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  •  Representative Standard Curve using ab133036.

Protocols

References

ab133036 has not yet been referenced specifically in any publications.

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