Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.7 pg/ml
- Range: 7.8 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Product nameProtein A ELISA Kit
Intra-assay Sample n Mean SD CV% Overall 5 3.7% Inter-assay Sample n Mean SD CV% Overall 3 10.2%
Sample typeCell culture supernatant
Assay typeSandwich (quantitative)
Range7.8 pg/ml - 500 pg/ml
Assay time1h 30m
Assay durationOne step assay
Protein A ELISA Kit (ab215542) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Protein A protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Protein A with 3.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Protein A (Staphylococcus aureus Protein A) is a 42 kDa protein that is characterized by its ability to bind the Fc portion of immunoglobulins.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Protein A Capture Antibody 1 x 600µl 10X Protein A Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BC 1 x 6ml Plate Seals 1 unit Protein A Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
RelevanceProtein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Cellular localizationSecreted; cell wall.
- gG-binding protein A
- Immunoglobulin G binding protein A
- Staphylococcal protein A
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of Protein A were measured in duplicate and interpolated from the Protein A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Protein A concentration was determined to be 442 pg/mL in the Rabbit Monoclonal Antibody sample.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
ab215542 has not yet been referenced specifically in any publications.