Overview

  • Product name

    Protein A Magnetic Beads
  • Sample type

    Serum, Cell Lysate
  • Species reactivity

    Reacts with: Mouse, Rabbit, Horse, Guinea pig, Hamster, Cow, Human, Pig
    Does not react with: Rat, Sheep, Goat, Chicken
  • Product overview

    Features:


    Easy to use, high-binding capacity, non-adherent beads.


    Support Characteristics: Paramagnetic, spherical, 6 % cross-linked agarose.


    Ligand: Recombinant Protein A.


    Particle Size: 75 – 150 µm.


    Binding Capacity: Generally >25 mg human IgG/ml wet beads.


    Working Temperature: Room temperature.


    Storage Solution: PBS with 0.02% Sodium Azide.


    Storage Temperature: 4 – 8 °C.


    Applications:


    Useful for immunoprecipitation and enrichment of IgG antibodies.


    High affinity for Fc region of IgG antibodies from a variety of species.


    Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3).


    It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.


     

  • Notes

    Description:

    Protein A Magnetic Beads are prepared by covalently coupling Recombinant Protein A to 6% crosslinked magnetically beaded agarose. The coupling technique is optimized to give a high binding capacity for IgG. The capacity of IgG binding is generally greater than 25 mg of human IgG per ml of wet gel.

     

  • Tested applications

    Suitable for: Purification, IPmore details

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 ml
    Protein A magnetic beads 1 x 1ml

Applications

Our Abpromise guarantee covers the use of ab214286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Purification Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

References

This product has been referenced in:

  • Wei L  et al. Novel urokinase-plasminogen activator inhibitor SPINK13 inhibits growth and metastasis of hepatocellular carcinoma in vivo. Pharmacol Res 143:73-85 (2019). Read more (PubMed: 30862605) »
  • Meng D  et al. MicroRNA-645 targets urokinase plasminogen activator and decreases the invasive growth of MDA-MB-231 triple-negative breast cancer cells. Onco Targets Ther 11:7733-7743 (2018). Read more (PubMed: 30464522) »
See all 2 Publications for this product

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