Product nameProtein A Magnetic Beads
Sample typeSerum, Cell Lysate
Species reactivityReacts with: Mouse, Rabbit, Horse, Guinea pig, Hamster, Cow, Human, Pig
Does not react with: Rat, Sheep, Goat, Chicken
Easy to use, high-binding capacity, non-adherent beads.
Support Characteristics: Paramagnetic, spherical, 6 % cross-linked agarose.
Ligand: Recombinant Protein A.
Particle Size: 75 – 150 µm.
Binding Capacity: Generally >25 mg human IgG/ml wet beads.
Working Temperature: Room temperature.
Storage Solution: PBS with 0.02% Sodium Azide.
Storage Temperature: 4 – 8 °C.
Useful for immunoprecipitation and enrichment of IgG antibodies.
High affinity for Fc region of IgG antibodies from a variety of species.
Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3).
It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
Protein A Magnetic Beads are prepared by covalently coupling Recombinant Protein A to 6% crosslinked magnetically beaded agarose. The coupling technique is optimized to give a high binding capacity for IgG. The capacity of IgG binding is generally greater than 25 mg of human IgG per ml of wet gel.
Tested applicationsSuitable for: Purification, IPmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 ml Protein A magnetic beads 1 x 1ml
Our Abpromise guarantee covers the use of ab214286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Purification||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab214286 has been referenced in 2 publications.
- Wei L et al. Novel urokinase-plasminogen activator inhibitor SPINK13 inhibits growth and metastasis of hepatocellular carcinoma in vivo. Pharmacol Res 143:73-85 (2019). PubMed: 30862605
- Meng D et al. MicroRNA-645 targets urokinase plasminogen activator and decreases the invasive growth of MDA-MB-231 triple-negative breast cancer cells. Onco Targets Ther 11:7733-7743 (2018). PubMed: 30464522