Overview

  • Product name

    Protein A/G/L Sepharose® Column
  • Product overview

    Contents:

    Supplied as ready-to-use pre-packed columns of 1 mL or 5 mL bead volume in PBS with 0.02% sodium azide.

     

    Features:

    Protein A/G/L binds to all IgG subclasses from various mammalian species that bind to Protein A, Protein G, and Protein L, making it the ideal choice for purification of a wide range of polyclonal or monoclonal IgG antibodies. The binding capacity is greater than 10 mg/mL of bead; high flow rate; low falling off of rProtein A/G/L; pH stability 2-10.

    Store column at 4°C.

    The beads may be damaged above 40°C.

    DO NOT FREEZE.

    Wash beads 3 times with 3x bead volume of desired buffer before use.

     

    Applications:

    Purification of monoclonal and polyclonal antibodies.

     

    Sepharose is a registered trademark of GE Healthcare

  • Notes

    Protein A/G/L-Sepharose® is prepared by covalently coupling recombinant Protein A/G/L (containing five Ig-binding regions from Protein L, five Ig-binding domains from Protein A, and three Ig-binding regions from Protein G) to 6% cross-linked Sepharose® beads. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure maximum specific IgG binding. The coupling technique is optimized to give a high binding capacity. The capacity of IgG binding could be greater than 10 mg of human IgG per ml of wet bead.

  • Tested applications

    Suitable for: Purificationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab193265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Purification Use at an assay dependent concentration.

Purification of monoclonal and polyclonal antibodies.

Protocols

References

ab193265 has not yet been referenced specifically in any publications.

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