Product nameProtein Block
Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
Protein blocking buffer for serum-free blocking of non-specific antibody binding in IHC, ELISA and western blot. No mixing or dilution required.
ab64226 reduces the handling of animal serums in the laboratory. The need to match species with the secondary antibody is eliminated due to the lack of normal serum in this product. It has been shown to be effective for immunohistochemistry, ELISA, and blot and requires no mixing or diluting.
Find more IHC kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Immunohistochemistry Incubate tissue section for 5 minutes at room temperature prior to application of the primary antibody. After incubation rinse once in buffer.
ELISA Add Protein Block to microtiter well and incubate for 2-10 minutes prior to addition of sample. Rinse and continue procedure.
Chemiluminescent Blot Protein Block has been reported to be an effective blocker for this technique with incubation times of one hour at room temperature or overnight at 4°C.
Storage instructionsStore at +4°C.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.5% BSA, 0.5% Casein, PBS
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Reagent notesab64226 reduces the handling of animal serums in the laboratory. The need to match species with the secondary antibody is eliminated due to the lack of normal serum in this product. It has been shown to be effective for immunohistochemistry, ELISA, and blot and requires no mixing or diluting.
RelevanceProtein Block is used with immunolabeling techniques for the reduction of non-specific background staining.
Our Abpromise guarantee covers the use of ab64226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution.|
ab64226 was used as pasr of the ab93677 anti-Mouse and Rabbit specific HRP (ABC) Detection IHC kit to perform immunohistochemical analysis of Human Tonsil tissue labeling Ki-67.
ab64226 was used in the mouse and rabbit specific HRP/DAB detection IHC Kit (ab64264) to visualize Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1:450 dilution.
This product has been referenced in:
- Botti G et al. COX-2 expression positively correlates with PD-L1 expression in human melanoma cells. J Transl Med 15:46 (2017). Read more (PubMed: 28231855) »
- Skardal A et al. Bioprinted amniotic fluid-derived stem cells accelerate healing of large skin wounds. Stem Cells Transl Med 1:792-802 (2012). IHC-Fr . Read more (PubMed: 23197691) »