Overview

  • Product name

    Anti-Protein C antibody [6B2]
    See all Protein C primary antibodies
  • Description

    Mouse monoclonal [6B2] to Protein C
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IHC-P, ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) from human plasma.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 2.9% Sodium chloride, 0.0268% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    6B2
  • Myeloma

    x63-Ag8.653
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
ELISA 1/30000.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 52 kDa.

Target

  • Function

    Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
  • Tissue specificity

    Plasma; synthesized in the liver.
  • Involvement in disease

    Thrombophilia due to protein C deficiency, autosomal dominant
    Thrombophilia due to protein C deficiency, autosomal recessive
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
    N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
  • Cellular localization

    Secreted. Golgi apparatus. Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activation peptide antibody
    • Anticoagulant protein C antibody
    • APC antibody
    • Autoprothrombin IIA antibody
    • Blood coagulation factor XIV antibody
    • EC 3.4.21.69 antibody
    • PC antibody
    • proC antibody
    • PROC_HUMAN antibody
    • PROC1 antibody
    • Protein C (inactivator of coagulation factors Va and VIIIa) antibody
    • THPH3 antibody
    • THPH4 antibody
    • Vitamin K dependent protein C antibody
    see all

Images

  • Paraffin-embedded Human liver tissue immunostained with ab36407

  • ICC/IF image of ab36407 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36407, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab36407 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Blocking
Sample
Human Cell lysate - whole cell (platelet rich plasma)
Specification
platelet rich plasma

Abcam user community

Verified customer

Submitted Mar 05 2019

Question
Answer

I'm happy to help! Please let me know if you need anything further.

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Answer

Thank you for your enquiry.

All of these antibodies are raised against full length Protein C. So we do not know what epitope(s) will be recognized by these antibodies.

I hope this is helpful. Please contact us again if you have any further questions.

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