Overview

  • Product name

  • Description

    Rabbit polyclonal to Protein C
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a sequence at the C terminal of Human Protein C.

  • Positive control

    • Jurkat, CEM, SMMC and Hela cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1-3µg for 106 cells.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 52 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
  • Tissue specificity

    Plasma; synthesized in the liver.
  • Involvement in disease

    Thrombophilia due to protein C deficiency, autosomal dominant
    Thrombophilia due to protein C deficiency, autosomal recessive
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
    N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
  • Cellular localization

    Secreted. Golgi apparatus. Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activation peptide antibody
    • Anticoagulant protein C antibody
    • APC antibody
    • Autoprothrombin IIA antibody
    • Blood coagulation factor XIV antibody
    • EC 3.4.21.69 antibody
    • PC antibody
    • proC antibody
    • PROC_HUMAN antibody
    • PROC1 antibody
    • Protein C (inactivator of coagulation factors Va and VIIIa) antibody
    • THPH3 antibody
    • THPH4 antibody
    • Vitamin K dependent protein C antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Protein C using ab131251 at 1μg/ml in human mammary cancer tissues. 
    Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
     
  • Overlay histogram showing A549 cells stained with ab131251 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with ab131251 1μg/1x106 cells for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG 5-10 μg/1x106 cells was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

  • All lanes : Anti-Protein C antibody (ab131251) at 0.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : CEM cell lysate
    Lane 3 : SMMC cell lysate
    Lane 4 : Hela cell lysate

    Predicted band size: 52 kDa

References

ab131251 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (HEK293T)
Specification
HEK293T
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 23 2014

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