Anti-Protein C antibody (ab131251)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Protein C antibody
See all Protein C primary antibodies -
Description
Rabbit polyclonal to Protein C -
Host species
Rabbit -
Specificity
No cross reactivity with other proteins. -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a sequence at the C terminal of Human Protein C.
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Positive control
- Jurkat, CEM, SMMC and Hela cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1-3µg for 106 cells. | |
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 52 kDa. | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845). -
Tissue specificity
Plasma; synthesized in the liver. -
Involvement in disease
Thrombophilia due to protein C deficiency, autosomal dominant
Thrombophilia due to protein C deficiency, autosomal recessive -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide. -
Cellular localization
Secreted. Golgi apparatus. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 5624 Human
- Omim: 612283 Human
- SwissProt: P04070 Human
- Unigene: 224698 Human
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Alternative names
- Activation peptide antibody
- Anticoagulant protein C antibody
- APC antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein C antibody (ab131251) -
Flow Cytometry - Anti-Protein C antibody (ab131251)Overlay histogram showing A549 cells stained with ab131251 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with ab131251 1μg/1x106 cells for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG 5-10 μg/1x106 cells was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Western blot - Anti-Protein C antibody (ab131251)All lanes : Anti-Protein C antibody (ab131251) at 0.5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : CEM cell lysate
Lane 3 : SMMC cell lysate
Lane 4 : Hela cell lysate
Predicted band size: 52 kDa
Protocols
References
ab131251 has not yet been referenced specifically in any publications.
