Product nameAnti-Protein C antibody
See all Protein C primary antibodies
DescriptionRabbit polyclonal to Protein C
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Protein C (internal sequence). The exact sequence is proprietary.
Database link: P04070
- WB: HepG2 and HCT 116 whole cell lysates. ICC/IF: HeLa cells. IHC-P: Human breast cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1% BSA, 20% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 52 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionProtein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
Tissue specificityPlasma; synthesized in the liver.
Involvement in diseaseThrombophilia due to protein C deficiency, autosomal dominant
Thrombophilia due to protein C deficiency, autosomal recessive
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
modificationsThe vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
Cellular localizationSecreted. Golgi apparatus. Endoplasmic reticulum.
- Information by UniProt
- Activation peptide antibody
- Anticoagulant protein C antibody
- APC antibody
All lanes : Anti-Protein C antibody (ab226998) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
10% SDS PAGE gel.
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Protein C (red) using ab226998 at 1/500 dilution in ICC/IF.
Paraffin embedded human breast cancer tissue stained for Protein C using ab226998 at 1/250 dilution in immunohistochemical analysis.
ab226998 has not yet been referenced specifically in any publications.