Key features and details
- Rabbit polyclonal to Protein C
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Protein C antibody
See all Protein C primary antibodies
DescriptionRabbit polyclonal to Protein C
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Protein C (internal sequence). The exact sequence is proprietary.
Database link: P04070
- WB: HepG2 and HCT 116 whole cell lysates. ICC/IF: HeLa cells. IHC-P: Human breast cancer tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 52 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionProtein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
Tissue specificityPlasma; synthesized in the liver.
Involvement in diseaseThrombophilia due to protein C deficiency, autosomal dominant
Thrombophilia due to protein C deficiency, autosomal recessive
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
modificationsThe vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
Cellular localizationSecreted. Golgi apparatus. Endoplasmic reticulum.
- Information by UniProt
- Activation peptide antibody
- Anticoagulant protein C antibody
- APC antibody
All lanes : Anti-Protein C antibody (ab226998) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 52 kDa
10% SDS PAGE gel.
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Protein C (red) using ab226998 at 1/500 dilution in ICC/IF.
Paraffin embedded human breast cancer tissue stained for Protein C using ab226998 at 1/250 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226998 has not yet been referenced specifically in any publications.