Overview

  • Product name

  • Description

    Rabbit polyclonal to Protein C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Protein C (internal sequence). The exact sequence is proprietary.
    Database link: P04070

  • Positive control

    • WB: HepG2 and HCT 116 whole cell lysates. ICC/IF: HeLa cells. IHC-P: Human breast cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1% BSA, 20% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
  • Tissue specificity

    Plasma; synthesized in the liver.
  • Involvement in disease

    Thrombophilia due to protein C deficiency, autosomal dominant
    Thrombophilia due to protein C deficiency, autosomal recessive
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
    N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
  • Cellular localization

    Secreted. Golgi apparatus. Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activation peptide antibody
    • Anticoagulant protein C antibody
    • APC antibody
    • Autoprothrombin IIA antibody
    • Blood coagulation factor XIV antibody
    • EC 3.4.21.69 antibody
    • PC antibody
    • proC antibody
    • PROC_HUMAN antibody
    • PROC1 antibody
    • Protein C (inactivator of coagulation factors Va and VIIIa) antibody
    • THPH3 antibody
    • THPH4 antibody
    • Vitamin K dependent protein C antibody
    see all

Images

  • All lanes : Anti-Protein C antibody (ab226998) at 1/1000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 52 kDa



    10% SDS PAGE gel.

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Protein C (red) using ab226998 at 1/500 dilution in ICC/IF.

  • Paraffin embedded human breast cancer tissue stained for Protein C using ab226998 at 1/250 dilution in immunohistochemical analysis.

References

ab226998 has not yet been referenced specifically in any publications.

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