Recombinant
RabMAb

Anti-Protein C antibody [EPSISR8] (ab150373)

Overview

  • Product name
    Anti-Protein C antibody [EPSISR8]
    See all Protein C primary antibodies
  • Description
    Rabbit monoclonal [EPSISR8] to Protein C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Protein C aa 300-400. The exact sequence is proprietary.

  • Positive control
    • Human plasma and HepG2 cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPSISR8
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab150373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
    • Tissue specificity
      Plasma; synthesized in the liver.
    • Involvement in disease
      Thrombophilia due to protein C deficiency, autosomal dominant
      Thrombophilia due to protein C deficiency, autosomal recessive
    • Sequence similarities
      Belongs to the peptidase S1 family.
      Contains 2 EGF-like domains.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 1 peptidase S1 domain.
    • Post-translational
      modifications
      The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
      N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
      The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
      May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
    • Cellular localization
      Secreted. Golgi apparatus. Endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activation peptide antibody
      • Anticoagulant protein C antibody
      • APC antibody
      • Autoprothrombin IIA antibody
      • Blood coagulation factor XIV antibody
      • EC 3.4.21.69 antibody
      • PC antibody
      • proC antibody
      • PROC_HUMAN antibody
      • PROC1 antibody
      • Protein C (inactivator of coagulation factors Va and VIIIa) antibody
      • THPH3 antibody
      • THPH4 antibody
      • Vitamin K dependent protein C antibody
      see all

    Images

    • All lanes : Anti-Protein C antibody [EPSISR8] (ab150373) at 1/1000 dilution

      Lane 1 : Human plasma lysates
      Lane 2 : HepG2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa

    References

    ab150373 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150373.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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