Recombinant Anti-Protein C antibody [EPSISR8] (ab150373)
![Western blot - Anti-Protein C antibody [EPSISR8] (ab150373)](https://www.abcam.com/ps/products/150/ab150373/Images/ab150373-156954-anti-protein-c-antibody-epsisr8-western-blot.jpg "Western blot - Anti-Protein C antibody [EPSISR8] (ab150373)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPSISR8] to Protein C
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Protein C antibody [EPSISR8]
See all Protein C primary antibodies -
Description
Rabbit monoclonal [EPSISR8] to Protein C -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Protein C aa 300-400. The exact sequence is proprietary.
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Positive control
- Human plasma and HepG2 cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPSISR8 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab150373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
Target
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Function
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845). -
Tissue specificity
Plasma; synthesized in the liver. -
Involvement in disease
Thrombophilia due to protein C deficiency, autosomal dominant
Thrombophilia due to protein C deficiency, autosomal recessive -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide. -
Cellular localization
Secreted. Golgi apparatus. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 5624 Human
- Omim: 612283 Human
- SwissProt: P04070 Human
- Unigene: 224698 Human
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Alternative names
- Activation peptide antibody
- Anticoagulant protein C antibody
- APC antibody
see all
Images
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Western blot - Anti-Protein C antibody [EPSISR8] (ab150373)All lanes : Anti-Protein C antibody [EPSISR8] (ab150373) at 1/1000 dilution
Lane 1 : Human plasma lysates
Lane 2 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa -
![Anti-Protein C antibody [EPSISR8] (ab150373)](https://www.abcam.com/ps/products/150/ab150373/Images/ab150373-2-benefits-of-recombinant-antibodies.png)
Anti-Protein C antibody [EPSISR8] (ab150373)
Datasheets and documents
References (0)
ab150373 has not yet been referenced specifically in any publications.
