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Hello, Iam very intersting in the dealing with your product in the abcam company, but I have a question about your product ( ab126287) Protein carbonyl content Assay Kit, the wave length that I must use it to measure optical density is ˜3750 and this according to the instruction in this kit, my problem is the readers in my university dont have the filter for this wavelength, the most closest one 405 wave length so my question is can I use 405 wave length to read the result for this Kit (ab126287).
please I need the answer as soon as possible
thank you very much
Asked on Jan 24 2013
Thank you for your message.
Every plate reader has flexibility in the band width detection range. If the reader being used has a flexibility of ± 30, you can use 345 to 405 nm for the 375 nm recommendation. Otherwise it might compromise the efficiency of the detection.
I hope this information is helpful. If you have any further questions, please do not hesitate to contact me.
Answered on Jan 24 2013