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Can this kit be used on mouse tissue? How is the tissue best prepared and what is the minimum tissue weight/protein concentration required
Asked on May 20 2013
This kit is species independent therefore mouse tissue can be used.
You can start with a minimum of 10 mg tissue and lyse it in any generic lysis buffer of your choice. We suggest washing the tissue 3 times with cold saline and blotting with filter paper. Weigh tissue and homogenize in potassium phosphate buffer 10 mM pH 7.4. Centrifuge at 10,000 RPM for 15 minutes at 4C, and use supernatant in the assay.
Answered on May 20 2013