Protein Carbonyl Content Assay Kit (Fluorometric) (ab235631)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Serum
- Sensitivity: 1 mg/ml
Overview
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Product name
Protein Carbonyl Content Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Cell Lysate -
Assay type
Quantitative -
Sensitivity
1 mg/ml -
Species reactivity
Reacts with: Human -
Product overview
Protein Carbonyl Content Assay Kit (Fluorometric) (ab235631) uses Fluoroscein-5-Thiosemicarbazide (FTC), a fluorescent probe which covalently reacts with oxidized residues (i.e. Cysteine, Lysine, Arginine, Histidine and Aspartic Acid) on proteins.
Protein carbonyl content is determined by the generation of a stable fluorometric signal (Ex/Em 485/535 nm), which is compared to the protein concentration determined in a BCA Assay to quantitate nmoles of carbonyl/mg protein.
The fluorometric protein carbonyl content assay kit is simple, requires no harsh chemicals, can quantitate carbonyls in serum or plasma and produces more reliable and reproducible results than the comparable colorimetric assays. It can detect carbonyl groups in samples with protein concentrations as low as 1 mg/mL.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K563 Protein Carbonyl Content Assay Kit (Fluorometric). K563-100 is the same size as the 100 test size of ab235631.
Related products
Other Protein Carbonyl Content assays include:
- Protein Carbonyl Content Assay (DNPH) ab126287 (most popular protein carbonyl content assay)
- Protein Carbonyl Content ELISA (DNPH) ab238536
- Protein Carbonyl Content Western Blot Assay ab178020Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Protein Carbonyl Assay Buffer 1 x 20ml 100% TCA Solution 1 x 20ml Streptozocin Solution 1 x 1ml Guanidine Solution 1 x 20ml Carbonyl Sample Dilution Buffer 1 x 50ml Fluoroscein-5-Thiosemicarbazide (FTC) 1 x 200µl AGE-BSA Positive Control Protein 1 x 250µl
Associated products
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Related Products
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab235631 has been referenced in 3 publications.
- Zhang L et al. Graphene oxide induces dose-dependent lung injury in rats by regulating autophagy. Exp Ther Med 21:462 (2021). PubMed: 33747194
- Olivares-González L et al. Nutraceutical Supplementation Ameliorates Visual Function, Retinal Degeneration, and Redox Status in rd10 Mice. Antioxidants (Basel) 10:N/A (2021). PubMed: 34206804
- Hoffman JF & Kalinich JF Effects of Incubation of Human Brain Microvascular Endothelial Cells and Astrocytes with Pyridostigmine Bromide, DEET, or Permethrin in the Absence or Presence of Metal Salts. Int J Environ Res Public Health 17:N/A (2020). PubMed: 33187257