Protein Carbonyl Content Assay Kit (Fluorometric) (ab235631)

Overview

  • Product name

    Protein Carbonyl Content Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Serum, Plasma, Cell Lysate
  • Assay type

    Quantitative
  • Sensitivity

    1 mg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Protein Carbonyl Content Assay Kit (Fluorometric) (ab235631) uses Fluoroscein-5-Thiosemicarbazide (FTC), a fluorescent probe which covalently reacts with oxidized residues (i.e. Cysteine, Lysine, Arginine, Histidine and Aspartic Acid) on proteins.


    Protein carbonyl content is determined by the generation of a stable fluorometric signal (Ex/Em 485/535 nm), which is compared to the protein concentration determined in a BCA Assay to quantitate nmoles of carbonyl/mg protein.


    The fluorometric protein carbonyl content assay kit is simple, requires no harsh chemicals, can quantitate carbonyls in serum or plasma and produces more reliable and reproducible results than the comparable colorimetric assays. It can detect carbonyl groups in samples with protein concentrations as low as 1 mg/mL.

  • Notes

    Related products

    Other Protein Carbonyl Content assays include:
    Protein Carbonyl Content Assay (DNPH) ab126287 (most popular protein carbonyl content assay)
    Protein Carbonyl Content ELISA (DNPH) ab238536
    Protein Carbonyl Content Western Blot Assay ab178020

    Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Protein Carbonyl Assay Buffer 1 x 20ml
    100% TCA Solution 1 x 20ml
    10% Streptozotocin Solution 1 x 1ml
    6 M Guanidine Solution 1 x 20ml
    Sample Dilution Buffer 1 x 50ml
    FTC (10 mM in DMSO) 1 x 200µl
    Positive Control Protein (10 mg/ml) 1 x 250µl

Images

  • 50 µL of human serum from patients with Alzheimer’s Disease (AD) or healthy individuals were assayed following kit protocols.

Protocols

References

ab235631 has not yet been referenced specifically in any publications.

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