Overview

  • Product name

    Protein Carbonyl ELISA Kit
    See all Protein Carbonyl kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Lysate
  • Assay type

    Cell-based (quantitative)
  • Product overview

    Protein Carbonyl ELISA Kit (ab238536) is designed for the quantitative measurement of protein carbonyls in plasma, serum, cell lysates or purified proteins. 


    With this kit protein samples are first allowed to adsorb to wells of a 96-well plate and then react with dinitrophenylhydrazine (DNPH), which allows for derivatization of the carbonyl group. There are no concentration or precipitation steps, that contribute to sample loss. The kit allows detection of as little as 10 µg/mL using a standard microplate reader. The quantity of protein carbonyls in protein sample is determined by comparing its absorbance with that of a known reduced/oxidized BSA standard curve.

  • Notes

    Related products

    Other Protein Carbonyl Content assays include:
    - Protein Carbonyl Content Assay (DNPH) ab126287 (most popular protein carbonyl content assay)
    - Protein Carbonyl Content Assay (Fluorometric) ab235631
    - Protein Carbonyl Content Western Blot Assay ab178020

    Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 96 tests 32 tests
    10X Wash Buffer 1 x 100ml 1 x 30ml
    25X DNPH Solution 1 x 500µl 1 x 500µl
    2X DNPH Diluent 1 x 15ml 1 x 15ml
    Protein Binding Strip Well Plate 1 unit 1 unit
    Anti-DNP Antibody (1000X) 1 x 20µl 1 x 5µl
    Blocking Reagent 1 x 20g 1 x 20g
    Oxidized BSA Standard 1 x 200µl 1 x 75µl
    Reduced BSA Standard 1 x 200µl 1 x 75µl
    Secondary Antibody, HRP Conjugate (1000X) 1 x 20µl 1 x 20µl
    Stop Solution 1 x 12ml 1 x 4ml
    Substrate Solution 1 x 12ml 1 x 4ml
  • Research areas

Images

  • This standard curve is for demonstration only. A standard curve must be run with each protein carbonyl elisa experiment.

  • STO (MEF), HeLa and MDA-231 cells were sonicated in 25mM HEPES, pH 7.5, 150 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol. Cell Lysates and BSA Standard from Pierce BCA Protein Assay were diluted to 10 μg/mL with 1X PBS and coated onto a 96-well Protein Binding Plate. The protein carbonyl levels were determined as described in the Protein Carbonyl ELISA Protocol.

Protocols

References

ab238536 has not yet been referenced specifically in any publications.

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