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    protein-casp-antibody-2a10-ab54583.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families Other
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Anti-Protein CASP antibody [2A10] (ab54583)

  • Datasheet
Reviews (9)Q&A (1)References (13)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] (ab54583)
  • Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [2A10] (ab54583)
  • Flow Cytometry - Anti-Protein CASP antibody [2A10] (ab54583)
  • Western blot - Anti-Protein CASP antibody [2A10] (ab54583)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] (ab54583)
  • Western blot - Anti-Protein CASP antibody [2A10] (ab54583)
  • Sandwich ELISA - Anti-Protein CASP antibody [2A10] (ab54583)

Key features and details

  • Mouse monoclonal [2A10] to Protein CASP
  • Suitable for: IHC-P, WB, ICC/IF, Sandwich ELISA, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Protein CASP antibody [2A10]
    See all Protein CASP primary antibodies
  • Description

    Mouse monoclonal [2A10] to Protein CASP
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Human Protein CASP aa 521-620. NP_001904.2
    Sequence:

    AENRLAQHTLQALQSELDSLRADNIKLFEKIKFLQSYPGRGSGSDDTELR YSSQYEERLDPFSSFSKRERQRKYLSLSPWDKATLSMGRLVLSNKMARTI


    Database link: Q13948
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC/IF: HeLa cells. IHC-P: Human spleen tissue; human malignant lymphoma, diffuse large B tissue. Flow Cytometry: MCF7 cells. WB: Protein CASP transfected HEK-293T cell lysate. Recombinant Protein CASP protein.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 13th Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    2A10
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab54583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
WB
Human
Recombinant fragment
Application Abreviews Notes
IHC-P (1)
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration.
ICC/IF (3)
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Notes
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    May be involved in intra-Golgi retrograde transport.
  • Sequence similarities

    Belongs to the CASP family.
  • Cellular localization

    Golgi apparatus membrane.
  • Target information above from: UniProt accession Q13948 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1523 Human
    • SwissProt: Q13948 Human
    • Unigene: 191482 Human
    • Alternative names

      • CASP_HUMAN antibody
      • CUX1 antibody
      • Protein CASP antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] (ab54583)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] (ab54583)

      Formalin-fixed, paraffin-embedded human spleen tissue stained for Protein CASP with ab54583 at 5 µg/ml in immunohistochemical analysis.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [2A10] (ab54583)
      Immunocytochemistry/ Immunofluorescence - Anti-Protein CASP antibody [2A10] (ab54583)

      HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Protein CASP (green) using ab54583 at 10 µg/ml in ICC/IF.

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-Protein CASP antibody [2A10] (ab54583)
      Flow Cytometry - Anti-Protein CASP antibody [2A10] (ab54583)

      Overlay histogram showing MCF7 (human breast adenocarcinoma cell line) cells stained with ab54583 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3 M glycine to block non-specific protein-protein interactions followed by the antibody (ab54583, 1 µg/1 x 106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1 x 106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-Protein CASP antibody [2A10] (ab54583)
      Western blot - Anti-Protein CASP antibody [2A10] (ab54583)
      All lanes : Anti-Protein CASP antibody [2A10] (ab54583) at 5 µg/ml

      Lane 1 : Protein CASP transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
      Lane 2 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate


      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] (ab54583)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein CASP antibody [2A10] (ab54583)

      Formalin-fixed, paraffin-embedded human malignant lymphoma, diffuse large B tissue stained for Protein CASP with ab54583 at 5 µg/ml in immunohistochemical analysis.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-Protein CASP antibody [2A10] (ab54583)
      Western blot - Anti-Protein CASP antibody [2A10] (ab54583)
      Anti-Protein CASP antibody [2A10] (ab54583) at 5 µg/ml + Tagged recombinant Protein CASP protein


      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

      Expected MW 38 kDa.

      This image was generated using the ascites version of the product.

    • Sandwich ELISA - Anti-Protein CASP antibody [2A10] (ab54583)
      Sandwich ELISA - Anti-Protein CASP antibody [2A10] (ab54583)

      Detection limit of ab54583 is approximately 0.03 ng/ml as a capture antibody.

      This image was generated using the ascites version of the product.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (13)

    Publishing research using ab54583? Please let us know so that we can cite the reference in this datasheet.

    ab54583 has been referenced in 13 publications.

    • Andrews MG  et al. mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex. Elife 9:N/A (2020). PubMed: 32876565
    • Oosterhof N  et al. Homozygous Mutations in CSF1R Cause a Pediatric-Onset Leukoencephalopathy and Can Result in Congenital Absence of Microglia. Am J Hum Genet 104:936-947 (2019). PubMed: 30982608
    • Giandomenico SL  et al. Cerebral organoids at the air-liquid interface generate diverse nerve tracts with functional output. Nat Neurosci 22:669-679 (2019). PubMed: 30886407
    • Zhang Z  et al. METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation. Cell Res N/A:N/A (2018). IHC-Fr ; Mouse . PubMed: 30297870
    • Edri R  et al. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun 6:6500 (2015). Other ; Human . PubMed: 25799239
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 10 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Protein CASP antibody [2A10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (iPSC-derived cortical neurons DIV90)
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    iPSC-derived cortical neurons DIV90
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 06 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Protein CASP antibody [2A10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (cortex)
    Permeabilization
    Yes - 3% Triton x100
    Specification
    cortex
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 26 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Protein CASP antibody [2A10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (cortical neurons)
    Permeabilization
    Yes - 0.25% Triton X-100 in PBS
    Specification
    cortical neurons
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 06 2018

    Immunohistochemistry free floating abreview for Anti-Protein CASP antibody [2A10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (P12 brain sections)
    Specification
    P12 brain sections
    Read More

    Dr. Bhavin Shah

    Verified customer

    Submitted Apr 17 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Protein CASP antibody [2A10]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rabbit Tissue sections (brain)
    Permeabilization
    Yes - 0.3% triton
    Specification
    brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 28 2017

    Immunocytochemistry abreview for Anti-Protein CASP antibody [2A10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (HeLa)
    Permeabilization
    Yes - 0.3% Triton-X-100 in PBS
    Specification
    HeLa
    Blocking step
    10% normal serum, 1% BSA in PBST as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 02 2013

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Protein CASP antibody [2A10]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Read More

    Dr. Sophie Pezet

    Verified customer

    Submitted Jun 08 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Protein CASP antibody [2A10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (brain, placenta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 20 min, 100C
    Permeabilization
    No
    Specification
    brain, placenta
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 09 2011

    Question

    Dear Sir or Madam,   I am interested in Anti-CUTL1 antibody and have some questions about it. There are 5 types anti-CUTL1 antibodies in the webpage. Anti-CUTL1 antibody (ab73885) looks better according to WB. What is the antibody's charaterization? Is it manufactured in-house or from outside labs? According to the reference, one of the antibodies is from xxxxxxxx group. Is ab73885 the right one?    Many Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 18 2011

    Answer

    Thank you for contacting us and sorry for the delay in getting back to you. We have a number of collaborators which produce antibodies for us as well as in house production of antibodies. The 5 antibodies that we have against CUTL1 have all been sourced through collaborators. All of the antibodies have been characterised through Western blotting and ab54583 and ab11584 has also been used in IHC. The diffferences observed in the western blots may be due to the antibodies being produced against different isoforms of CULT1. Whilst ab73885, ab85994 and ab98273 have been raised against immunogen sequences from CULT1 isoform 1 (SwissProt P39880) and would be expected to react with all CULT1 isoforms, ab115854 and ab54583 have both been raised against an immunogen sequence taken from CULT1 isoform 4 (SwissProt Q13948). These would therefore be expected to recognise isoform 4 and isoform 8 of CULT1 only (expected at around 77 kDa). I'm sorry if this information may not have been made explicit on the datasheet. Unfortunately I have no knowledge of any of the antibodies being produced by the group mentioned. You have not clarified the application or type of sample intended with the antibody but ab73885 would be a good choice if intending to perform Western blotting or ELISA with human CUTL1. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 18 2011

    Western blot abreview for Anti-Protein CASP antibody [2A10]

    Poor
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MDA-MB-231, HT-1080, NIH3T3, D283Med, Daoy)
    Gel Running Conditions
    Reduced Denaturing (8%gel)
    Loading amount
    500000 cells
    Specification
    MDA-MB-231, HT-1080, NIH3T3, D283Med, Daoy
    Blocking step
    3% non fat milk/ 2% BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Ms. Sabine Topka

    Verified customer

    Submitted Oct 07 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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