Overview

  • Product name
    Anti-Protein cornichon homolog 2 antibody - N-terminal
  • Description
    Rabbit polyclonal to Protein cornichon homolog 2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Protein cornichon homolog 2 aa 38-67 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6PI25

  • Positive control
    • Human brain tissue; HL60 lysate; HL60 and U251MG cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170290 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 19 kDa.
ICC/IF 1/10 - 1/50.

Target

  • Function
    Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by regulating their rates of activation, deactivation and desensitization. Blocks CACNG8-mediated resensitization of AMPA receptors.
  • Sequence similarities
    Belongs to the cornichon family.
  • Cellular localization
    Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendrite. Cell projection > dendritic spine. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Also localizes to the cell membrane of extrasynaptic sites (dendritic shafts, spines of pyramidal cells).
  • Information by UniProt
  • Database links
  • Alternative names
    • CNI like antibody
    • CNIH 2 antibody
    • CNIH2 antibody
    • CNIH2_HUMAN antibody
    • Cnih3 antibody
    • Cnil antibody
    • Cornichon homolog 2 (Drosophila) antibody
    • Cornichon homolog 2 antibody
    • Cornichon like protein antibody
    • Cornichon, Drosophila, homolog of, 2 antibody
    • Cornichon-like protein antibody
    • Protein cornichon homolog 2 antibody
    • RGD1304930 antibody
    see all

Images

  • Flow cytometric analysis of HL60 cells labeling Protein cornichon homolog 2 with ab170290 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram) . FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Confocal immunofluorescent analysis of U251MG cells labeling Protein cornichon homolog 2 with ab170290 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling Protein cornichon homolog 2 with ab170290 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Anti-Protein cornichon homolog 2 antibody - N-terminal (ab170290) at 1/100 dilution + HL60 cell line lysate at 35 µg

    Predicted band size: 19 kDa

References

ab170290 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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