Overview

  • Product name

    Anti-Protein G antibody (Biotin)
    See all Protein G primary antibodies
  • Description

    Rabbit polyclonal to Protein G (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: ELISAmore details
  • Immunogen

    Full length native protein (purified) corresponding to Protein G. Strepococcus species

  • Positive control

    • Natural Protein G (ab7458) can be used as a positive control in WB.
  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab7251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
  • Cellular localization

    Cell Wall and Secreted
  • Alternative names

    • IgG binding protein G antibody
    • IgG-binding protein G antibody
    • Immunoglobulin G binding protein G [Precursor] antibody
    • spg antibody
    • spg immunoglobulin G-binding protein antibody
    • SPG2_STRSG antibody
    see all

References

ab7251 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Sample
Staphylococcus aureus Cell (human T cells)
Permeabilization
No
Gating Strategy
Singlets gating (linear cells on FCS-H and FSC-A plot).
Specification
human T cells
Preparation
Cell harvesting/tissue preparation method: Live Jurkat T cells grown in 10% FBS RPMI
Sample buffer: cells and proteins allowed to bind in DPBS + 1% BSA + 1 mM EDTA. 0.2 micron filtered.
Fixation
Cells were kept live on ice throughout experiment

Abcam user community

Verified customer

Submitted Jun 13 2019

Question
Answer

The answer is no, it is not epitope mapped. The antibody is polyclonal and raised against the whole protein. It regonizes multiple epitopes.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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