Key features and details
- Rabbit polyclonal to Protein G (Biotin)
- Suitable for: ELISA
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-Protein G antibody (Biotin)
See all Protein G primary antibodies
DescriptionRabbit polyclonal to Protein G (Biotin)
Tested applicationsSuitable for: ELISAmore details
Full length native protein (purified) corresponding to Protein G. Strepococcus species
- Natural Protein G (ab7458) can be used as a positive control in WB.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
Concentration information loading...
Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
Our Abpromise guarantee covers the use of ab7251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
RelevanceProtein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
Cellular localizationCell Wall and Secreted
- IgG binding protein G antibody
- IgG-binding protein G antibody
- Immunoglobulin G binding protein G [Precursor] antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab7251 has not yet been referenced specifically in any publications.