Protein G Sepharose® (ab193259)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Protein G Sepharose® -
Sample type
Cell culture supernatant, Serum, Cell culture media, Ascites Fluid -
Product overview
Contents:
Supplied as 50% slurry in 20% Ethanol.
Features:
High binding capacity: Binding of IgG >20 mg human or rabbit IgG/mL Protein G Sepharose®.
Minimal Leaching of the Ligand
Flow Rate Tested*: 2.07 mL/min.
*Test condition: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).
Usage: Reusable for up to 10 times without significant loss of binding capacity.These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.Sepharose is a registered trademark of GE Healthcare
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Notes
Protein G Sepharose® beads are prepared by covalently coupling recombinant Protein G to 6% cross-linked Sepharose® beads. Protein G is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG and minimum leaching of recombinant Protein G. The IgG binding capacity of Protein G Sepharose® is >20 mg of human or rabbit IgG per mL of wet beads. Protein G Sepharose® beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.
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Tested applications
Suitable for: Purification, IPmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 5 ml 25 ml 100 ml Protein G Sepharose® 1 x 1ml 1 x 5ml 1 x 25ml 1 x 100ml -
Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab193259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Purification | Use at an assay dependent concentration. Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. |
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| IP | Use at an assay dependent concentration. Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. |
References
This product has been referenced in:
- DeLeo FR et al. Survival of Carbapenem-Resistant Klebsiella pneumoniae Sequence Type 258 in Human Blood. Antimicrob Agents Chemother 61:N/A (2017). Human . Read more (PubMed: 28115349) »
