Product nameAnti-Protein Kinase A regulatory subunit I alpha/PRKAR1A antibody [EPR8491]
See all Protein Kinase A regulatory subunit I alpha/PRKAR1A primary antibodies
DescriptionRabbit monoclonal [EPR8491] to Protein Kinase A regulatory subunit I alpha/PRKAR1A
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Protein Kinase A regulatory subunit I alpha/PRKAR1A aa 200-300. The exact sequence is proprietary.
- Recombinant Human Protein Kinase A regulatory subunit I alpha/PRKAR1A (ab125532) can be used as a positive control in WB. Human brain tissue; Human fetal heart, 293T and U-87 MG lysates.
This product was previously labelled as Protein Kinase A regulatory subunit I alpha
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab139695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 43 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended.
Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Involvement in diseaseDefects in PRKAR1A are the cause of Carney complex type 1 (CNC1) [MIM:160980]. CNC is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas.
Defects in PRKAR1A are the cause of intracardiac myxoma (INTMYX) [MIM:255960]. Inheritance is autosomal recessive.
Defects in PRKAR1A are the cause of primary pigmented nodular adrenocortical disease type 1 (PPNAD1) [MIM:610489]. Primary pigmented nodular adrenocortical disease is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. PPNAD1 is most often diagnosed in patients with Carney complex, but it can also be observed in patients without other manifestations or familial history.
Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.
modificationsThe pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
- Information by UniProt
- ACRDYS1 antibody
- ADOHR antibody
- cAMP dependent protein kinase regulatory subunit alpha 1 antibody
All lanes : Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A antibody [EPR8491] (ab139695) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : 293T cell lysate
Lane 3 : U-87 MG cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 43 kDa
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Protein Kinase A regulatory subunit I alpha/PRKAR1A, with ab139695 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- He J et al. Recurrent somatic mutations ofPRKAR1Ain isolated cardiac myxoma. Oncotarget 8:103968-103974 (2017). Read more (PubMed: 29262613) »
- Wang S et al. PRKAR1A is a functional tumor suppressor inhibiting ERK/Snail/E-cadherin pathway in lung adenocarcinoma. Sci Rep 6:39630 (2016). IHC-P ; Human . Read more (PubMed: 27995993) »