Product nameAnti-Protein Kinase A regulatory subunit I alpha/PRKAR1A (phospho S77) antibody [EPMAYR1-127]
See all Protein Kinase A regulatory subunit I alpha/PRKAR1A primary antibodies
DescriptionRabbit monoclonal [EPMAYR1-127] to Protein Kinase A regulatory subunit I alpha/PRKAR1A (phospho S77)
ab139682 only detects Protein Kinase A regulatory subunit I alpha/PRKAR1A phosphorylated at serine 77.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Protein Kinase A regulatory subunit I alpha/PRKAR1A.
- Fetal heart tissue lysate.
This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Ozgur Ogut at the Mayo Clinic.
This product was previously labelled as Protein Kinase A regulatory subunit I alpha
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851)
- Anti-Protein Kinase A regulatory subunit I alpha antibody [EPMAYR1-127] - BSA and Azide free (ab248879)
- Anti-Protein Kinase A regulatory subunit I alpha antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
Our Abpromise guarantee covers the use of ab139682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 43 kDa.|
|IP||1/10 - 1/100.|
Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Involvement in diseaseDefects in PRKAR1A are the cause of Carney complex type 1 (CNC1) [MIM:160980]. CNC is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas.
Defects in PRKAR1A are the cause of intracardiac myxoma (INTMYX) [MIM:255960]. Inheritance is autosomal recessive.
Defects in PRKAR1A are the cause of primary pigmented nodular adrenocortical disease type 1 (PPNAD1) [MIM:610489]. Primary pigmented nodular adrenocortical disease is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. PPNAD1 is most often diagnosed in patients with Carney complex, but it can also be observed in patients without other manifestations or familial history.
Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.
modificationsThe pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
- Information by UniProt
- ACRDYS1 antibody
- ADOHR antibody
- cAMP dependent protein kinase regulatory subunit alpha 1 antibody
All lanes : Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A (phospho S77) antibody [EPMAYR1-127] (ab139682) at 1/1000 dilution
Lane 1 : Fetal heart lysate
Lane 2 : Fetal heart lysate treated with Alkaline Phosphatase.
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
ab139682 has not yet been referenced specifically in any publications.