Recombinant
RabMAb

Recombinant Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A (phospho S77) antibody [EPMAYR1-127] (ab139682)

Overview

  • Product name

    Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A (phospho S77) antibody [EPMAYR1-127]
    See all Protein Kinase A regulatory subunit I alpha/PRKAR1A primary antibodies
  • Description

    Rabbit monoclonal [EPMAYR1-127] to Protein Kinase A regulatory subunit I alpha/PRKAR1A (phospho S77)
  • Host species

    Rabbit
  • Specificity

    ab139682 only detects Protein Kinase A regulatory subunit I alpha/PRKAR1A phosphorylated at serine 77.

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Protein Kinase A regulatory subunit I alpha/PRKAR1A.

  • Positive control

    • Fetal heart tissue lysate.
  • General notes

    This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Ozgur Ogut at the Mayo Clinic.

     This product was previously labelled as Protein Kinase A regulatory subunit I alpha

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab139682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Tissue specificity

      Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
    • Involvement in disease

      Defects in PRKAR1A are the cause of Carney complex type 1 (CNC1) [MIM:160980]. CNC is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas.
      Defects in PRKAR1A are the cause of intracardiac myxoma (INTMYX) [MIM:255960]. Inheritance is autosomal recessive.
      Defects in PRKAR1A are the cause of primary pigmented nodular adrenocortical disease type 1 (PPNAD1) [MIM:610489]. Primary pigmented nodular adrenocortical disease is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the adrenals is characteristic with small pigmented micronodules observed in the cortex. PPNAD1 is most often diagnosed in patients with Carney complex, but it can also be observed in patients without other manifestations or familial history.
    • Sequence similarities

      Belongs to the cAMP-dependent kinase regulatory chain family.
      Contains 2 cyclic nucleotide-binding domains.
    • Post-translational
      modifications

      The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACRDYS1 antibody
      • ADOHR antibody
      • cAMP dependent protein kinase regulatory subunit alpha 1 antibody
      • cAMP dependent protein kinase regulatory subunit RIalpha antibody
      • cAMP dependent protein kinase type I alpha regulatory chain antibody
      • cAMP dependent protein kinase type I alpha regulatory subunit antibody
      • cAMP-dependent protein kinase type I-alpha regulatory subunit antibody
      • CAR antibody
      • Carney complex type 1 antibody
      • CNC 1 antibody
      • CNC antibody
      • CNC1 antibody
      • DKFZp779L0468 antibody
      • KAP0_HUMAN antibody
      • MGC17251 antibody
      • PKA RIA antibody
      • PKR 1 antibody
      • PKR1 antibody
      • PPNAD 1 antibody
      • PPNAD1 antibody
      • PRKAR 1 antibody
      • PRKAR1 antibody
      • PRKAR1A antibody
      • PRKAR1A/RARA FUSION GENE antibody
      • Protein kinase A type 1a regulatory subunit antibody
      • Protein kinase cAMP dependent regulatory type I alpha antibody
      • PTC2 CHIMERIC ONCOGENE, INCLUDED antibody
      • Tissue specific extinguisher 1 antibody
      • Tissue-specific extinguisher 1 antibody
      • TSE 1 antibody
      • TSE1 antibody
      see all

    Images

    • All lanes : Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A (phospho S77) antibody [EPMAYR1-127] (ab139682) at 1/1000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : Fetal heart lysate treated with Alkaline Phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 43 kDa

    References

    ab139682 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab139682.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up